MTFR1 Polyclonal antibody

• Featured Product
• KD/KO Validated

MTFR1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 17778-1-AP

Synonyms

CHPPR, FAM54A2, KIAA0009, MTFR1

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Validation Data Gallery

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  WB analysis using 17778-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17778-1-AP (MTFR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 17778-1-AP

  WB result of MTFR1 antibody (17778-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MTFR1 transfected A549 cells.

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  IP experiment of mouse testis using 17778-1-AP

  IP result of anti-MTFR1 (IP:17778-1-AP, 4ug; Detection:17778-1-AP 1:800) with mouse testis tissue lysate 4800ug.

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Tested Applications

• Positive WB detected in: A549 cells, HCT 116 cells, HepG2 cells, SW480 cells
• Positive IP detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17778-1-AP targets MTFR1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MTFR1 fusion protein Ag10857
• Full Name: mitochondrial fission regulator 1
• Calculated Molecular Weight: 333 aa, 37 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC036116
• Gene Symbol: MTFR1
• Gene ID (NCBI): 9650
• RRID: AB_2282301
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MTFR1, also known as CHPPR, is a mitochondria protein. It may play a role in mitochondrial aerobic respiration. It may also regulate mitochondrial organization and fission.