Tested Applications
| Positive WB detected in | A549 cells, HCT 116 cells, HepG2 cells, SW480 cells |
| Positive IP detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17778-1-AP targets MTFR1 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10857 Product name: Recombinant human MTFR1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-333 aa of BC036116 Sequence: MLGWIKRLIRMVFQQVGVSMQSVLWSRKPYGSSRSIVRKIGTNLSLIQCPRVQFQINSHATEWSPSHPGEDAVASFADVGWVAKEEGECSARLRTEVRSRPPLQDDLLFFEKAPSRQISLPDLSQEEPQLKTPARANEEALQKICALENELAALRAQIAKIVTQQEQQNLTAGDLDSTTFGTIPPHPPPPPPPLPPPALGLHQSTSAVDLIKERREKRANAGKTLVKNNPKKPEMPNMLEILKEMNSVKLRSVKRSEQDVKPKPVDATDPAALIAEALKKKFAYRYRSDSQDEVEKGIPKSESEATSERVLFGPHMLKPTGKMKALIENVSDS Predict reactive species |
| Full Name | mitochondrial fission regulator 1 |
| Calculated Molecular Weight | 333 aa, 37 kDa |
| Observed Molecular Weight | 37 kDa |
| GenBank Accession Number | BC036116 |
| Gene Symbol | MTFR1 |
| Gene ID (NCBI) | 9650 |
| RRID | AB_2282301 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15390 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MTFR1, also known as CHPPR, is a mitochondria protein. It may play a role in mitochondrial aerobic respiration. It may also regulate mitochondrial organization and fission.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for MTFR1 antibody 17778-1-AP | Download protocol |
| WB protocol for MTFR1 antibody 17778-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-MTFR1 antibody (Cat# 17778-1-AP) has 4 total citations published in Science Advances, Neoplasia, IUBMB Life, and IET Systems Biology. The research spans mitochondrial biology—covering mitochondrial distribution and fusion—and oncology, including colon cancer survival under glucose deprivation, triple-negative breast cancer proliferation/metastasis, and MTFR1 as a prognostic biomarker in breast cancer. Applications include WB, IHC, and CoIP in human and monkey samples.
| Species | Application | Title |
|---|---|---|
Sci Adv ARMC1 partitions between distinct complexes and assembles MIRO with MTFR to control mitochondrial distribution | ||
Neoplasia MTFR1 phosphorylation-activated adaptive mitochondrial fusion is essential for colon cancer cell survival during glucose deprivation
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IUBMB Life MTFR1 Promotes Proliferation and Metastasis of Triple-Negative Breast Cancer by Regulating Mitochondrial Metabolism.
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IET Syst Biol Identification of MTFR1 as a Novel Prognostic Biomarker and Putative Oncogene for Breast Cancer: A Multi-Omics Analysis and in Vitro Experimental Validation.
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