MTHFD2 Monoclonal antibody, PBS Only

MTHFD2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B4B6

Cat no : 68524-1-PBS

Synonyms

MTHFD2

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Validation Data Gallery

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  WB analysis using 68524-1-Ig (same clone as 68524-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68524-1-Ig (MTHFD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68524-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 68524-1-Ig (same clone as 68524-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68524-1-Ig (MTHFD2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68524-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 68524-1-Ig (same clone as 68524-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68524-1-Ig (MTHFD2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68524-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 68524-1-Ig (same clone as 68524-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68524-1-Ig (MTHFD2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68524-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 68524-1-Ig (same clone as 68524-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68524-1-Ig (MTHFD2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68524-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68524-1-PBS targets MTHFD2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: MTHFD2 fusion protein Ag33605
• Full Name: methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase
• Calculated Molecular Weight: 350 aa, 38 kDa
• Observed Molecular Weight: 33-38 kDa
• GenBank Accession Number: BC017054
• Gene Symbol: MTHFD2
• Gene ID (NCBI): 10797
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Methylenetetrahydrofolate dehydrogenase 2 (MTHFD2) is a folate-coupled mitochondrial metabolic enzyme characterized by methylenetetrahydrofolate dehydrogenase and cyclohydrolase activity. It was demonstrated that suppression of MTHFD2 inhibits methylation reactions, decreases protein synthesis and disrupts redox homeostasis, which may ultimately result in significant changes in cellular metabolic phenotype. MTHFD2 is reported to be up‐regulated in various tumours, such as lung adenocarcinoma(PMID: 34121323) and breast cancer(PMID: 34007312).