MTHFD2 Recombinant monoclonal antibody, PBS Only

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• MTHFD2 Recombinant monoclonal antibody, PBS Only (86608-3-PBS)

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  WB analysis using 86608-3-RR (same clone as 86608-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86608-3-RR (MTHFD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86608-3-PBS in a different storage buffer formulation.

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  WB analysis of rat testis using 86608-3-RR (same clone as 86608-3-PBS)

  rat testis tissue were subjected to SDS PAGE followed by western blot with 86608-3-RR (MTHFD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86608-3-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 86608-3-RR (same clone as 86608-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MTHFD2 antibody (86608-3-RR, Clone: 251431F2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86608-3-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 86608-3-RR (same clone as 86608-3-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug MTHFD2 Recombinant monoclonal antibody (86608-3-RR, Clone:251431F2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 86608-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86608-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86608-3-RR against Human MTHFD2 were performed. The affinity constant is 2.95 nM.

MTHFD2 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 86608-3-PBS

Clone No.251431F2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial, Methenyltetrahydrofolate cyclohydrolase

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Product Information

86608-3-PBS targets MTHFD2 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag2911

  Product name: Recombinant human MTHFD2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-350 aa of BC017054

  Sequence: MAATSLMSALAARLLQPAHSCSLRLRPFHLAAVRNEAVVISGRKLAQQIKQEVRQEVEEWVASGNKRPHLSVILVGENPASHSYVLNKTRAAAVVGINSETIMKPASISEEELLNLINKLNNDDNVDGLLVQLPLPEHIDERRICNAVSPDKDVDGFHVINVGRMCLDQYSMLPATPWGVWEIIKRTGIPTLGKNVVVAGRSKNVGMPIAMLLHTDGAHERPGGDATVTISHRYTPKEQLKKHTILADIVISAAGIPNLITADMIKEGAAVIDVGINRVHDPVTAKPKLVGDVDFEGVRQKAGYITPVPGGVGPMTVAMLMKNTIIAAKKVLRLEEREVLKSKELGVATN

• Full Name: methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase
• Calculated Molecular Weight: 350 aa, 38 kDa
• Observed Molecular Weight: 33-38 kDa
• GenBank Accession Number: BC017054
• Gene Symbol: MTHFD2
• Gene ID (NCBI): 10797
• RRID: AB_3745003
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P13995
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Methylenetetrahydrofolate dehydrogenase 2 (MTHFD2) is a folate-coupled mitochondrial metabolic enzyme characterized by methylenetetrahydrofolate dehydrogenase and cyclohydrolase activity. It was demonstrated that suppression of MTHFD2 inhibits methylation reactions, decreases protein synthesis and disrupts redox homeostasis, which may ultimately result in significant changes in cellular metabolic phenotype. MTHFD2 is reported to be up‐regulated in various tumours, such as lung adenocarcinoma(PMID: 34121323) and breast cancer(PMID: 34007312).