MTIF3 Polyclonal antibody

• Featured Product
• KD/KO Validated

MTIF3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

8

Reviews (1)

Cat no : 14219-1-AP

Synonyms

IF 3(Mt), IF 3mt, IF3(mt), IF3mt, MTIF3

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Validation Data Gallery

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  WB analysis using 14219-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14219-1-AP (MTIF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human preadipocyte cells using 14219-1-AP

  The MTIF3 knockout (>80% KO efficiency as evaluated by Sanger sequencing) and wildtype (WT) human preadipocyte cells were lysed in 1% SDS. 10 μg total protein were subjected to SDS PAGE followed by western blot with 14219-1-AP (MTIF3 antibody) at dilution of 1:2000 incubated overnight at 4 oC. Date from Dr. Mi Huang.

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  IP experiment of HeLa using 14219-1-AP

  IP result of anti-MTIF3 (IP:14219-1-AP, 4ug; Detection:14219-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IHC staining of human liver using 14219-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14219-1-AP (MTIF3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 14219-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14219-1-AP (MTIF3 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 14219-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14219-1-AP (MTIF3 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 14219-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14219-1-AP (MTIF3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 14219-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14219-1-AP (MTIF3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 14219-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14219-1-AP (MTIF3 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: A431 cells, human preadipocyte cells, HT-29 cells, HeLa cells, HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human liver tissue, mouse testis tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below

Product Information

14219-1-AP targets MTIF3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MTIF3 fusion protein Ag5457
• Full Name: mitochondrial translational initiation factor 3
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC046166
• Gene Symbol: MTIF3
• Gene ID (NCBI): 219402
• RRID: AB_10638621
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MTIF3, also named as DC38, belongs to the IF-3 family. MTIF3 encodes a 29 kDa protein that promotes formation of the initiation complex on the mitochondrial 55S ribosome, thereby playing an active role in initiation of translation. Like bacterial IF3, MTIF3 is believed to bind first to the small mitoribosomal subunit to keep it dissociated from the large subunit during initiation. After binding of MTIF3, mRNA and formylated initiator methionyl-tRNA (fMet-tRNAifMet) bind to the small mitoribosomal subunit. The large subunit then joins the small subunit to form an elongation-competent ribosome (PMID:20887776, 31350787).

Publications

Species	Application	Title
mouse	WB	Sci Adv; Fidelity of translation initiation is required for coordinated respiratory complex assembly.; Authors - Danielle L Rudler
mouse	WB	EMBO J; Stress signaling and cellular proliferation reverse the effects of mitochondrial mistranslation.; Authors - Nicola Ferreira
human	WB	Sci Rep; Initiation Factor 3 is Dispensable For Mitochondrial Translation in Cultured Human Cells.; Authors - Ivan V Chicherin
		Clin Immunol; Novel minor HLA DR associated antigens in type 1 diabetes.; Authors - Denise Müller
rat	WB	Sci Total Environ; Fluoride impairs mitochondrial translation by targeting miR-221-3p/c-Fos/RMND1 axis contributing to neurodevelopment defects; Authors - Dongjie Li
human	WB	Elife; Identification of a weight loss-associated causal eQTL in MTIF3 and the effects of MTIF3 deficiency on human adipocyte function; Authors - Mi Huang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Mi (Verified Customer) (02-10-2023)

It works pretty well! We tried different antibodies from other suppliers, and this one gives a specific band, as we expected from the knockout samples. Although, we did observe some non-specific bands, having a knockout control would validate the target band.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: adipocytes