MTMR3 Polyclonal antibody

MTMR3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 21336-1-AP

Synonyms

FYVE DSP1, KIAA0371, MTMR3, myotubularin related protein 3, ZFYVE10

---

Validation Data Gallery

• 

  WB analysis of SW 1990 using 21336-1-AP

  SW 1990 cells were subjected to SDS PAGE followed by western blot with 21336-1-AP (MTMR3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 21336-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21336-1-AP (MTMR3 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 21336-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21336-1-AP (MTMR3 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: SW 1990 cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 21336-1-AP is MTMR3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MTMR3 fusion protein Ag15514
• Full Name: myotubularin related protein 3
• Calculated Molecular Weight: 1198 aa, 134 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC151217
• Gene Symbol: MTMR3
• Gene ID (NCBI): 8897
• RRID: AB_2878842
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Mol Ther Nucleic Acids; SPI1-induced downregulation of FTO promotes GBM progression by regulating pri-miR-10a processing in an m6A-dependent manner.; Authors - Shouji Zhang