MTMR4 Polyclonal antibody

MTMR4 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13488-1-AP

Synonyms

myotubularin related protein 4, KIAA0647, FYVE-DSP2, FYVE DSP2, FYVE domain-containing dual specificity protein phosphatase 2

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Validation Data Gallery

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  WB analysis using 13488-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13488-1-AP (MTMR4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 13488-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MTMR4 antibody (13488-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells, MCF-7 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

13488-1-AP targets MTMR4 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MTMR4 fusion protein Ag4310
• Full Name: myotubularin related protein 4
• Calculated Molecular Weight: 1195 aa, 133 kDa
• Observed Molecular Weight: 133 kDa
• GenBank Accession Number: BC035609
• Gene Symbol: MTMR4
• Gene ID (NCBI): 9110
• RRID: AB_2251008
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Myotubularin-related protein-4 (MTMR4), also named FYVE-DSP2, is a member of the myotubularin family of phosphoinositide phosphatases that selectively degrade the 3-position phosphate from the inositol ring of PtdIns(3)P and phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) (PMID: 31543504). MTMR4 knockdown markedly suppressed the motility, fusion, and fission of PI(3)P- enriched structures, resulting in decreases in late endosomes, autophagosomes, and lysosomes, and enlargement of PI(3)P- enriched early and late endosomes(PMID: 29962048).

Publications

Species	Application	Title
mouse	WB	J Biol Chem; PtdIns3P phosphatases MTMR3 and MTMR4 negatively regulate innate immune responses to DNA through modulating STING trafficking.; Authors - Dyaningtyas Dewi Pamungkas Putri