Tested Applications
| Positive WB detected in | Jurkat cells, A549 cells, MCF-7 cells, HeLa cells, HepG2 cells |
| Positive IHC detected in | hepatocellular carcinoma tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13488-1-AP targets MTMR4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4310 Product name: Recombinant human MTMR4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 845-1195 aa of BC035609 Sequence: SHQEQLSSVPDLTHGEEDIGKRGNNRNGQLLENPRFGKMPLELVRKPISQSQISEFSFLGSNWDSFQGMVTSFPSGEATPRRLLSYGCCSKRPNSKQMRATGPCFGGQWAQREGVKSPVCSSHSNGHCTGPGGKNQMWLSSHPKQVSSTKPVPLNCPSPVPPLYLDDDGLPFPTDVIQHRLRQIEAGYKQEVEQLRRQVRELQMRLDIRHCCAPPAEPPMDYEDDFTCLKESDGSDTEDFGSDHSEDCLSEASWEPVDKKETEVTRWVPDHMASHCYNCDCEFWLAKRRHHCRNCGNVFCAGCCHLKLPIPDQQLYDPVLVCNSCYEHIQVSRARELMSQQLKKPIATASS Predict reactive species |
| Full Name | myotubularin related protein 4 |
| Calculated Molecular Weight | 1195 aa, 133 kDa |
| Observed Molecular Weight | 133-150 kDa |
| GenBank Accession Number | BC035609 |
| Gene Symbol | MTMR4 |
| Gene ID (NCBI) | 9110 |
| RRID | AB_2251008 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NYA4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Myotubularin-related protein-4 (MTMR4), also named FYVE-DSP2, is a member of the myotubularin family of phosphoinositide phosphatases that selectively degrade the 3-position phosphate from the inositol ring of PtdIns(3)P and phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) (PMID: 31543504). MTMR4 knockdown markedly suppressed the motility, fusion, and fission of PI(3)P- enriched structures, resulting in decreases in late endosomes, autophagosomes, and lysosomes, and enlargement of PI(3)P- enriched early and late endosomes(PMID: 29962048).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MTMR4 antibody 13488-1-AP | Download protocol |
| IHC protocol for MTMR4 antibody 13488-1-AP | Download protocol |
| WB protocol for MTMR4 antibody 13488-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MTMR4 Polyclonal antibody (Cat# 13488-1-AP) has 2 total citations. These appear in Cell Reports and the Journal of Biological Chemistry. The cited studies focus on autophagic lysosome reformation involving RAF1 kinase (human, WB) and MTMR3/MTMR4 regulation of innate immune responses to DNA via STING trafficking (mouse, WB). Both applications used Western blot, spanning cell biology and immunology research fields.
| Species | Application | Title |
|---|---|---|
J Biol Chem PtdIns3P phosphatases MTMR3 and MTMR4 negatively regulate innate immune responses to DNA through modulating STING trafficking. |
