MTMR4 Polyclonal antibody

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  WB analysis using 13488-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13488-1-AP (MTMR4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 13488-1-AP

  WB result of MTMR4 antibody (13488-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-MTMR4 transfected MCF-7 cells.

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  IHC staining of Hepatocellular carcinoma using 13488-1-AP

  Immunohistochemical analysis of paraffin-embedded hepatocellular carcinoma tissue slide using 13488-1-AP (MTMR4 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of Hepatocellular carcinoma using 13488-1-AP

  Immunohistochemical analysis of paraffin-embedded hepatocellular carcinoma tissue slide using 13488-1-AP (MTMR4 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 13488-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 13488-1-AP (MTMR4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 13488-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 13488-1-AP (MTMR4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 13488-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MTMR4 antibody (13488-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

MTMR4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 13488-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

EC:3.1.3.48, FYVE domain-containing dual specificity protein phosphatase 2, FYVE DSP2, FYVE-DSP2, KIAA0647

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Tested Applications

• Positive WB detected in: Jurkat cells, MCF-7 cells
• Positive IHC detected in: hepatocellular carcinoma tissue, rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

13488-1-AP targets MTMR4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4310

  Product name: Recombinant human MTMR4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 845-1195 aa of BC035609

  Sequence: SHQEQLSSVPDLTHGEEDIGKRGNNRNGQLLENPRFGKMPLELVRKPISQSQISEFSFLGSNWDSFQGMVTSFPSGEATPRRLLSYGCCSKRPNSKQMRATGPCFGGQWAQREGVKSPVCSSHSNGHCTGPGGKNQMWLSSHPKQVSSTKPVPLNCPSPVPPLYLDDDGLPFPTDVIQHRLRQIEAGYKQEVEQLRRQVRELQMRLDIRHCCAPPAEPPMDYEDDFTCLKESDGSDTEDFGSDHSEDCLSEASWEPVDKKETEVTRWVPDHMASHCYNCDCEFWLAKRRHHCRNCGNVFCAGCCHLKLPIPDQQLYDPVLVCNSCYEHIQVSRARELMSQQLKKPIATASS

• Full Name: myotubularin related protein 4
• Calculated Molecular Weight: 1195 aa, 133 kDa
• Observed Molecular Weight: 133-150 kDa
• GenBank Accession Number: BC035609
• Gene Symbol: MTMR4
• Gene ID (NCBI): 9110
• RRID: AB_2251008
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NYA4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Myotubularin-related protein-4 (MTMR4), also named FYVE-DSP2, is a member of the myotubularin family of phosphoinositide phosphatases that selectively degrade the 3-position phosphate from the inositol ring of PtdIns(3)P and phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) (PMID: 31543504). MTMR4 knockdown markedly suppressed the motility, fusion, and fission of PI(3)P- enriched structures, resulting in decreases in late endosomes, autophagosomes, and lysosomes, and enlargement of PI(3)P- enriched early and late endosomes(PMID: 29962048).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	J Biol Chem; PtdIns3P phosphatases MTMR3 and MTMR4 negatively regulate innate immune responses to DNA through modulating STING trafficking.; Authors - Dyaningtyas Dewi Pamungkas Putri
human	WB	Cell Rep; RAF1 kinase contributes to autophagic lysosome reformation; Authors - Stefanie Toifl