MTMR8 Polyclonal antibody

MTMR8 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12299-1-AP

Synonyms

Phosphatidylinositol-3-phosphate phosphatase, Phosphatidylinositol-3,5-bisphosphate 3-phosphatase, Myotubularin-related protein 8, myotubularin related protein 8, EC:3.1.3.95

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Validation Data Gallery

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  WB analysis of HeLa using 12299-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12299-1-AP (MTMR8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 12299-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12299-1-AP (MTMR8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12299-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12299-1-AP (MTMR8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 12299-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12299-1-AP (MTMR8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A375 using 12299-1-AP

  Immunofluorescent analysis of A375 cells using 12299-1-AP (MTMR8 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, A375 cells, human placenta tissue
• Positive IF/ICC detected in: A375 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12299-1-AP targets MTMR8 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MTMR8 fusion protein Ag2944
• Full Name: myotubularin related protein 8
• Calculated Molecular Weight: 704 aa, 79 kDa
• Observed Molecular Weight: 55-58 kDa
• GenBank Accession Number: BC012399
• Gene Symbol: MTMR8
• Gene ID (NCBI): 55613
• RRID: AB_2298075
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.