MTRR Polyclonal antibody

• 

  WB analysis of K-562 using 26944-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 26944-1-AP (MTRR Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary cancer using 26944-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 26944-1-AP (MTRR antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MTRR Polyclonal Antibody for WB, IHC, ELISA

Cat No. 26944-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Synonyms

MSR, Methionine synthase reductase, EC:1.16.1.8, cblE, Aquacobalamin reductase

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Tested Applications

• Positive WB detected in: K-562 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 2 publications below

Product Information

26944-1-AP targets MTRR in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag25678

  Product name: Recombinant human MTRR protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 376-725 aa of BC109216

  Sequence: TKKKGATLPQHIPAGCSLQFIFTWCLEIRAIPKKAFLRALVDYTSDSAEKRRLQELCSKQGAADYSRFVRDACACLLDLLLAFPSCQPPLSLLLEHLPKLQPRPYSCASSSLFHPGKLHFVFNIVEFLSTATTEVLRKGVCTGWLALLVASVLQPNIHASHEDSGKALAPKISISPRTTNSFHLPDDPSIPIIMVGPGTGIAPFIGFLQHREKLQEQHPDGNFGAMWLFFGCRHKDRDYLFRKELRHFLKHGILTHLKVSFSRDAPVGEEEAPAKYVQDNIQLHGQQVARILLQENGHIYVCGDAKNMAKDVHDALVQIISKEVGVEKLEAMKTLATLKEEKRYLQDIWS

• Full Name: 5-methyltetrahydrofolate-homocysteine methyltransferase reductase
• Calculated Molecular Weight: 725 aa, 80 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC109216
• Gene Symbol: MTRR
• Gene ID (NCBI): 4552
• RRID: AB_2880694
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UBK8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	ACS Cent Sci; Chlorquinaldol Alleviates Lung Fibrosis in Mice by Inhibiting Fibroblast Activation through Targeting Methionine Synthase Reductase; Authors - Xiangyu Yang
mouse	IHC	Front Cell Dev Biol; Disruption of Folate Metabolism Causes Poor Alignment and Spacing of Mouse Conceptuses for Multiple Generations.; Authors - Amy L Wilkinson
mouse	IHC	Reprod Fertil Dev; Analysis of spermatogenesis and fertility in adult mice with a hypomorphic mutation in the Mtrr gene; Authors - Georgina E T Blake