Tested Applications
| Positive WB detected in | K-562 cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26944-1-AP targets MTRR in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25678 Product name: Recombinant human MTRR protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 376-725 aa of BC109216 Sequence: TKKKGATLPQHIPAGCSLQFIFTWCLEIRAIPKKAFLRALVDYTSDSAEKRRLQELCSKQGAADYSRFVRDACACLLDLLLAFPSCQPPLSLLLEHLPKLQPRPYSCASSSLFHPGKLHFVFNIVEFLSTATTEVLRKGVCTGWLALLVASVLQPNIHASHEDSGKALAPKISISPRTTNSFHLPDDPSIPIIMVGPGTGIAPFIGFLQHREKLQEQHPDGNFGAMWLFFGCRHKDRDYLFRKELRHFLKHGILTHLKVSFSRDAPVGEEEAPAKYVQDNIQLHGQQVARILLQENGHIYVCGDAKNMAKDVHDALVQIISKEVGVEKLEAMKTLATLKEEKRYLQDIWS Predict reactive species |
| Full Name | 5-methyltetrahydrofolate-homocysteine methyltransferase reductase |
| Calculated Molecular Weight | 725 aa, 80 kDa |
| Observed Molecular Weight | 80 kDa |
| GenBank Accession Number | BC109216 |
| Gene Symbol | MTRR |
| Gene ID (NCBI) | 4552 |
| RRID | AB_2880694 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UBK8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MTRR antibody 26944-1-AP | Download protocol |
| WB protocol for MTRR antibody 26944-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MTRR Polyclonal antibody (Cat# 26944-1-AP) has a total of 4 citations. They appear in Advanced Science, ACS Central Science, Frontiers in Cell and Developmental Biology, and Reproduction, Fertility and Development. The associated research fields include pulmonary fibrosis, lung fibrosis, folate metabolism during embryonic development, and spermatogenesis/fertility, with applications in WB and IHC on mouse samples.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Homocysteine Exacerbates Pulmonary Fibrosis via Orchestrating Syntaxin 17 Homocysteinylation of Alveolar Type II Cells | ||
ACS Cent Sci Chlorquinaldol Alleviates Lung Fibrosis in Mice by Inhibiting Fibroblast Activation through Targeting Methionine Synthase Reductase | ||
Front Cell Dev Biol Disruption of Folate Metabolism Causes Poor Alignment and Spacing of Mouse Conceptuses for Multiple Generations. | ||
Reprod Fertil Dev Analysis of spermatogenesis and fertility in adult mice with a hypomorphic mutation in the Mtrr gene |
