MTSS1 Polyclonal antibody, PBS Only

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• MTSS1 Polyclonal antibody, PBS Only (13995-1-PBS)

• 

  WB analysis using 13995-1-AP (same clone as 13995-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13995-1-AP (MTSS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13995-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 13995-1-AP (same clone as 13995-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13995-1-AP (MTSS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13995-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 13995-1-AP (same clone as 13995-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13995-1-AP (MTSS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13995-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 13995-1-AP (same clone as 13995-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13995-1-AP (MTSS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13995-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 13995-1-AP (same clone as 13995-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13995-1-AP (MTSS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13995-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 13995-1-AP (same clone as 13995-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using MTSS1 antibody (13995-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13995-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

MTSS1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 13995-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

13995-1-PBS targets MTSS1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5080

  Product name: Recombinant human MTSS1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 126-473 aa of BC023998

  Sequence: SQDAFQSKSPSPMPPEAPNQRRKEKREPDPNGGGPTTASGPPAAAEEAQRPRSMTVSAATRPGEEMEACEELALALSRGLQLDTQRSSRDSLQCSSGYSTQTTTPCCSEDTIPSQVSDYDYFSVSGDQEADQQEFDKSSTIPRNSDISQSYRRMFQAKRPASTAGLPTTLGPAMVTPGVATIRRTPSTKPSVRRGTIGAGPIPIKTPVIPVKTPTVPDLPGVLPAPPDGPEERGEHSPESPSVGEGPQGVTSMPSSMWSGQASVNPPLPGPKPSIPEEHRQAIPESEAEDQEREPPSATVSPGQIPESDPADLSPRDTPQGEDMLNAIRRGVKLKKTTTNDRSAPRFS

• Full Name: metastasis suppressor 1
• Calculated Molecular Weight: 82 kDa
• Observed Molecular Weight: 82 kDa
• GenBank Accession Number: BC023998
• Gene Symbol: MTSS1
• Gene ID (NCBI): 9788
• RRID: AB_3085428
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43312
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

MTSS1 (metastasis suppressor 1), also known as MIM (missing-in-metastasis), MIM-B, BEG4 (Basal cell carcinoma-enriched gene 4) or KIAA0429, is one of the defining members of the I-BAR family of negative membrane curvature-sensing proteins first identified as being deleted in metastatic bladder cancer. MTSS1 over-expression is associated with enhanced cell migration, resulting in tumorigenesis, invasion and metastasis (PMID: 27230279). It is reported that the expression of MTSS1 is associated with poor prognosis in various cancers,such as bladder, gastric, breast and so on (PMID: 32052679).