Tested Applications
| Positive WB detected in | mouse liver tissue, mouse small intestine tissue, rat liver tissue, rat small intestine tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
23940-1-AP targets MTTP/MTP in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21045 Product name: Recombinant human MTTP protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-151 aa of BC062696 Sequence: MILLAVLFLCFISSYSASVKGHTTGLSLNNDRLYKLTYSTEVLLDRGKGKLQDSVGYRISSNVDVALLWRNPDGDDDQLIQITMKDVNVENVNQQRGEKSIFKGKSPSKIMGKENLEALQRPTLLHLIHGKVKGRLDSTTFSPTSYFSSLQ Predict reactive species |
| Full Name | microsomal triglyceride transfer protein |
| Calculated Molecular Weight | 151aa,17 kDa; 894aa,99 kDa |
| Observed Molecular Weight | 90-100 kDa |
| GenBank Accession Number | BC062696 |
| Gene Symbol | MTTP |
| Gene ID (NCBI) | 4547 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | P55157 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MTTP, also known as MTP, belongs to the lipid transfer protein family and forms a heterodimeric complex with protein disulfide isomerase (PDI) to perform lipid transfer functions. MTTP is isolated as a soluble protein from the lumen of a microsomal fraction of liver and intestine. MTTP assembles triglycerides and apoB in the liver and intestine and subsequently produces apoB-containing lipoproteins. Deficiency in MTTP can cause abetalipoproteinemia, characterized by the absence of apoB-containing lipoproteins from the circulatory system(PMID: 10946006, 23475612).
