Tested Applications
| Positive WB detected in | mouse heart tissue, mouse kidney tissue, rat heart tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11610-1-AP targets MTX2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2181 Product name: Recombinant human MTX2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-253 aa of BC017271 Sequence: MYIAAEPWPENATLYQQLKGEQILLSDNAASLAVQAFLQMCNLPIKVVCRANAEYMSPSGKVPFIHVGNQVVSELGPIVQFVKAKGHSLSDGLEEVQKAEMKAYMELVNNMLLTAELYLQWCDEATVGEITHARYGSPYPWPLNHILAYQKQWEVKRKMKAIGWGKKTLDQVLEDVDQCCQALSQRLGTQPYFFNKQPTELDALVFGHLYTILTTQLTNDELSEKVKNYSNLLAFCRRIEQHYFEDRGKGRLS Predict reactive species |
| Full Name | metaxin 2 |
| Calculated Molecular Weight | 263 aa, 30 kDa |
| Observed Molecular Weight | 25-29 kDa |
| GenBank Accession Number | BC017271 |
| Gene Symbol | MTX2 |
| Gene ID (NCBI) | 10651 |
| RRID | AB_10859665 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75431 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MTX2 antibody 11610-1-AP | Download protocol |
| IHC protocol for MTX2 antibody 11610-1-AP | Download protocol |
| IP protocol for MTX2 antibody 11610-1-AP | Download protocol |
| WB protocol for MTX2 antibody 11610-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MTX2 Polyclonal antibody (Cat# 11610-1-AP) has 8 citations published in journals including Cell Death & Disease, Hepatology, Cell Death & Differentiation, International Journal of Biological Sciences, NPJ Parkinson's Disease, Cell Reports, and Journal of Ovarian Research. Research fields span nasopharyngeal carcinoma, mitochondrial membrane remodeling and liver injury, mitochondrial cristae architecture, mitochondrial dysfunction and nephrotic proteinuria, Parkinson's disease axonal transport, mitophagy regulation, mtDNA release/inflammation, and oocyte aging.
| Species | Application | Title |
|---|---|---|
Cell Death Dis EB virus-induced ATR activation accelerates nasopharyngeal carcinoma growth via M2-type macrophages polarization. | ||
Hepatology Loss of Sam50 in hepatocytes induces cardiolipin-dependent mitochondrial membrane remodeling to trigger mtDNA release and liver injury | ||
Cell Death Differ Sam50-Mic19-Mic60 axis determines mitochondrial cristae architecture by mediating mitochondrial outer and inner membrane contact | ||
Int J Biol Sci Loss of MTX2 causes mitochondrial dysfunction, podocyte injury, nephrotic proteinuria and glomerulopathy in mice and patients
| ||
NPJ Parkinsons Dis Disruption of axonal transport in Parkinson's disease: the role of pathological α-Syn and AMPK/p38 MAPK signaling | ||
Cell Rep Sam50 Regulates PINK1-Parkin-Mediated Mitophagy by Controlling PINK1 Stability and Mitochondrial Morphology.
|
