Tested Applications
| Positive WB detected in | mouse heart tissue, mouse kidney tissue, rat heart tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11610-1-AP targets MTX2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2181 Product name: Recombinant human MTX2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-253 aa of BC017271 Sequence: MYIAAEPWPENATLYQQLKGEQILLSDNAASLAVQAFLQMCNLPIKVVCRANAEYMSPSGKVPFIHVGNQVVSELGPIVQFVKAKGHSLSDGLEEVQKAEMKAYMELVNNMLLTAELYLQWCDEATVGEITHARYGSPYPWPLNHILAYQKQWEVKRKMKAIGWGKKTLDQVLEDVDQCCQALSQRLGTQPYFFNKQPTELDALVFGHLYTILTTQLTNDELSEKVKNYSNLLAFCRRIEQHYFEDRGKGRLS Predict reactive species |
| Full Name | metaxin 2 |
| Calculated Molecular Weight | 263 aa, 30 kDa |
| Observed Molecular Weight | 25-29 kDa |
| GenBank Accession Number | BC017271 |
| Gene Symbol | MTX2 |
| Gene ID (NCBI) | 10651 |
| RRID | AB_10859665 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75431 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MTX2 antibody 11610-1-AP | Download protocol |
| IHC protocol for MTX2 antibody 11610-1-AP | Download protocol |
| IP protocol for MTX2 antibody 11610-1-AP | Download protocol |
| WB protocol for MTX2 antibody 11610-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MTX2 Polyclonal antibody (Cat# 11610-1-AP) has 8 citations published in journals including Cell Death & Disease, Hepatology, Cell Death & Differentiation, International Journal of Biological Sciences, npj Parkinson's Disease, Cell Reports (×2), and Journal of Ovarian Research. Research fields span nasopharyngeal carcinoma, liver injury, mitochondrial cristae architecture and dysfunction, nephrotic proteinuria, Parkinson's disease axonal transport, mitophagy, and oocyte aging.
| Species | Application | Title |
|---|---|---|
Cell Death Dis EB virus-induced ATR activation accelerates nasopharyngeal carcinoma growth via M2-type macrophages polarization. | ||
Hepatology Loss of Sam50 in hepatocytes induces cardiolipin-dependent mitochondrial membrane remodeling to trigger mtDNA release and liver injury | ||
Cell Death Differ Sam50-Mic19-Mic60 axis determines mitochondrial cristae architecture by mediating mitochondrial outer and inner membrane contact | ||
Int J Biol Sci Loss of MTX2 causes mitochondrial dysfunction, podocyte injury, nephrotic proteinuria and glomerulopathy in mice and patients
| ||
NPJ Parkinsons Dis Disruption of axonal transport in Parkinson's disease: the role of pathological α-Syn and AMPK/p38 MAPK signaling | ||
Cell Rep Mitochondrial cristae architecture protects against mtDNA release and inflammation
|
