MUC13 Polyclonal antibody

MUC13 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29832-1-AP

Synonyms

DRCC1, FLJ20063, MUC 13, MUC13, Mucin 13, RECC

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Validation Data Gallery

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  WB analysis using 29832-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29832-1-AP (MUC13 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 29832-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29832-1-AP (MUC13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 29832-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29832-1-AP (MUC13 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 29832-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29832-1-AP (MUC13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 29832-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29832-1-AP (MUC13 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 29832-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 29832-1-AP (MUC13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 29832-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 29832-1-AP (MUC13 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HCT 116 cells, HEK-293 cells, HT-29 cells, SW480 cells, mouse kidney tissue, rat kidney tissue
• Positive IHC detected in: human colon cancer tissue, human colon tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29832-1-AP targets MUC13 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MUC13 fusion protein Ag31090
• Full Name: mucin 13, cell surface associated
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 70-120 kDa
• GenBank Accession Number: NM_033049
• Gene Symbol: MUC13
• Gene ID (NCBI): 56667
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mucins (MUC) are highly glycosylated proteins that are abundantly expressed in mammalian epithelial cells. Mucin 13 (MUC13) is a member of the transmembrane glycoprotein mucins which is normally expressed in the large intestine, trachea, kidney, small intestine, and gastric epithelium (PMID: 22027689). MUC13 is involved in the tumorigenesis of multiple malignancies, including pancreatic cancer, colorectal cancer, renal cancer and lung cancer (PMID: 22027689; PMID: 31427737; PMID: 28205224; PMID: 34966453).