Tested Applications
| Positive WB detected in | human placenta tissue, PNGF treated human placenta tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33669-1-AP targets MUC15 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Eg5625 Product name: recombinant human MUC15 protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 24-236 aa of NM_145650 Sequence: KENQDINTTQNIAEVFKTMENKPISLESEANLNSDKENITTSNLKASHSPPLNLPNNSHGITDFSSNSSAEHSLGSLKPTSTISTSPPLIHSFVSKVPWNAPIADEDLLPISAHPNATPALSSENFTWSLVNDTVKTPDNSSITVSILSSEPTSPSVTPLIVEPSGWLTTNSDSFTGFTPYQEKTTLQPTLKFTNNSKLFPNTSDPQKENRNT Predict reactive species |
| Full Name | mucin 15, cell surface associated |
| Calculated Molecular Weight | 36 kDa |
| Observed Molecular Weight | 85 kD, 110 kD |
| GenBank Accession Number | NM_145650 |
| Gene Symbol | MUC15 |
| Gene ID (NCBI) | 143662 |
| RRID | AB_3743044 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q8N387-1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Mucin 15(MUC15) is a member of the transmembrane glycoprotein mucins that are abundantly expressed in the epithelial cells of mammals. MUC15 is involved in cell adhesion and signal transduction, and it plays a role in promoting or inhibiting tumor progression. (PMID: 12047385; 17720698;21615302)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MUC15 antibody 33669-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
