MUC4 Polyclonal antibody, PBS Only

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• MUC4 Polyclonal antibody, PBS Only (28703-1-PBS)

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  WB analysis using 28703-1-AP (same clone as 28703-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28703-1-AP (MUC4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28703-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 28703-1-AP (same clone as 28703-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28703-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 28703-1-AP (same clone as 28703-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28703-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 28703-1-AP (same clone as 28703-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28703-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 28703-1-AP (same clone as 28703-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28703-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 28703-1-AP (same clone as 28703-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28703-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 28703-1-AP (same clone as 28703-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28703-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 28703-1-AP (same clone as 28703-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28703-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 28703-1-AP (same clone as 28703-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28703-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 28703-1-AP (same clone as 28703-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28703-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 28703-1-AP (same clone as 28703-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28703-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 28703-1-AP (same clone as 28703-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28703-1-PBS in a different storage buffer formulation.

MUC4 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 28703-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Mucin 4, MUC-4, MUC 4, ASGP 2, ASGP 1 Mucin 4 beta chain

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Product Information

28703-1-PBS targets MUC4 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag29799

  Product name: Recombinant human MUC4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 29-225 aa of NM_018406

  Sequence: TTEDTLITGSKTAAPVTSTGSTTATLEGQSTAASSRTSNQDISASSQNHQTKSTETTSKAQTDTLTQMMTSTLFSSPSVHNVMETVTQETAPPDEMTTSFPSSVTNTLMMTSKTITMTTSTDSTLGNTEETSTAGTESSTPVTSAVSITAGQEGQSRTTSWRTSIQDTSASSQNHWTRSTQTTRESQTSTLTHRTTS

• Full Name: mucin 4, cell surface associated
• Calculated Molecular Weight: 231 kDa
• Observed Molecular Weight: 231 kDa, 113 kDa
• GenBank Accession Number: NM_018406
• Gene Symbol: MUC4
• Gene ID (NCBI): 4585
• RRID: AB_2918192
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99102
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

MUC4, also named as HSA276359, ASGP, MUC-4, testis mucin and mucin-4 is a high molecular weight transmembrane mucin that is overexpressed in many carcinomas and is a risk factor associated with a poor prognosis. MUC4 have a key role in the progression of pancreatic cancer. MUC4 helps in the docking of tumor cells on the endothelial surface.