MUC4 Polyclonal antibody

MUC4 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 55343-1-AP

Synonyms

Ascites sialoglycoprotein, Ascites sialoglycoprotein 1, Ascites sialoglycoprotein 2, ASGP, ASGP 1 Mucin 4 beta chain, ASGP 2, HSA276359, MUC 4, MUC4, Mucin 4, Testis mucin, Tracheobronchial mucin



Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

55343-1-AP targets MUC4 in WB, IHC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name mucin 4, cell surface associated
Calculated molecular weight 232 kDa
Observed molecular weight 235-250 kDa
GenBank accession numberNM_004532
Gene symbol MUC4
Gene ID (NCBI) 4585
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

MUC4, also named as HSA276359, ASGP, MUC-4, testis mucin and mucin-4 is a high molecular weight transmembrane mucin that is overexpressed in many carcinomas and is a risk factor associated with a poor prognosis. MUC4 have a key role in the progression of pancreatic cancer. MUC4 helps in the docking of tumor cells on the endothelial surface. This antibody is specific to MUC4.

Protocols

Product Specific Protocols
WB protocol for MUC4 antibody 55343-1-APDownload protocol
IHC protocol for MUC4 antibody 55343-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols