Tested Applications
| Positive IHC detected in | human stomach tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20725-1-AP targets MUC5AC in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | mucin 5AC, oligomeric mucus/gel-forming |
| Calculated Molecular Weight | 527 kDa |
| GenBank Accession Number | NM_017511 |
| Gene Symbol | MUC5AC |
| Gene ID (NCBI) | 4586 |
| RRID | AB_3669342 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P98088 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MUC5AC is a member of the mucin family, a heterogeneous group of 21 abundant, high molecular weight O-glycoproteins that can be either secreted or membrane bound. Multiple cysteine-rich domains in both N- and C-terminal regions of MUC5AC are responsible for its disulfide-mediated polymerization, which is critical for gel-forming properties. (PMID: 26751774, PMID: 27845339)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MUC5AC antibody 20725-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MUC5AC Polyclonal antibody (Cat# 20725-1-AP) has 13 citations published in journals including J Allergy Clin Immunol, J Nanobiotechnology, Phytomedicine, Antioxidants, Frontiers in Immunology, Drug Delivery and Translational Research, Journal of Ethnopharmacology, International Immunopharmacology, Molecular Immunology, Experimental and Therapeutic Medicine, Frontiers in Pediatrics, and Translational Pediatrics. Research fields span allergic airway inflammation, asthma, oxidative stress, ferroptosis, mucin biology, and gut-liver dysfunction.
| Species | Application | Title |
|---|---|---|
J Nanobiotechnology Palladium nanozyme loaded metal-organic framework eyedrops alleviate oxidative stress for dry eye treatment. | ||
J Allergy Clin Immunol Eosinophilic chronic rhinosinusitis with nasal polyps: Squamous metaplasia as an associated remodeling feature. | ||
Phytomedicine Genkwanin reduces airway epithelial cell ferroptosis and alleviates asthma symptoms in mice. | ||
Antioxidants (Basel) Cold Exposure Exacerbates Allergic Airway Inflammation via Ferroptosis: Evidence from a Murine Model. | ||
Front Immunol Nasopharyngeal swab transcriptomics identifies divergent molecular signatures in adenoid hypertrophy and allergic rhinitis comorbidity. | ||
Drug Deliv Transl Res M2 macrophage-derived small extracellular vesicles carrying canagliflozin attenuate experimental asthma. |
