MUC5AC Recombinant monoclonal antibody

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• MUC5AC Recombinant monoclonal antibody (85868-1-RR)

• 

  WB analysis using 85868-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85868-1-RR (MUC5AC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 85868-1-RR

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 85868-1-RR (MUC5AC antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 85868-1-RR

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 85868-1-RR (MUC5AC antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MUC5AC Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 85868-1-RR

Clone No.250061E6

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

MUC5

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Tested Applications

• Positive WB detected in: rat stomach tissue
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

85868-1-RR targets MUC5AC in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: mucin 5AC, oligomeric mucus/gel-forming
• Calculated Molecular Weight: 527 kDa
• Observed Molecular Weight: 290 kDa
• GenBank Accession Number: NM_017511
• Gene Symbol: MUC5AC
• Gene ID (NCBI): 4586
• RRID: AB_3744433
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P98088
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MUC5AC (Mucin-5AC ) is a gel-forming glycoprotein of the gastric and respiratory epithelium. It can protect mucous membranes from infection and chemical damage by binding to inhaled microorganisms and particles, which are then cleared by the mucociliary system. (PubMed:14535999,PubMed:14718370)

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	IHC	Mol Med Rep; Cigarette smoke combined with Klebsiella pneumoniae induce damage to the air‑blood barrier in chronic obstructive pulmonary disease rats via the MAPK/NF‑κB/IκBα pathway .; Authors - YANGE TIAN