MURC Polyclonal antibody

MURC Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 55464-1-AP

Synonyms

cavin‐4, MURC

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Validation Data Gallery

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  WB analysis of mouse heart using 55464-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55464-1-AP (MURC Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 55464-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 55464-1-AP (MURC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 55464-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 55464-1-AP (MURC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse heart tissue
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

55464-1-AP targets MURC in WB, IHC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: muscle-related coiled-coil protein
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 48-53 kDa
• GenBank Accession Number: NM_001018116
• Gene Symbol: MURC
• Gene ID (NCBI): 347273
• RRID: AB_2881343
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Nutrients; Dietary α-Linolenic Acid Counters Cardioprotective Dysfunction in Diabetic Mice: Unconventional PUFA Protection.; Authors - Jake S Russell