Tested Applications
| Positive WB detected in | HepG2 cells |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11018-1-AP targets MUS81 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1476 Product name: Recombinant human MUS81 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 202-551 aa of BC009999 Sequence: RYSLTPEGLELAQKLAESEGLSLLNVGIGPKEPPGEETAVPGAASAELASEAGVQQQPLELRPGEYRVLLCVDIGETRGGGHRPELLRELQRLHVTHTVRKLHVGDFVWVAQETNPRDPANPGELVLDHIVERKRLDDLCSSIIDGRFREQKFRLKRCGLERRVYLVEEHGSVHNLSLPESTLLQAVTNTQVIDGFFVKRTADIKESAAYLALLTRGLQRLYQGHTLRSRPWGTPGNPESGAMTSPNPLCSLLTFSDFNAGAIKNKAQSVREVFARQLMQVRGVSGEKAAALVDRYSTPASLLAAYDACATPKEQETLLSTIKCGRLQRNLGPALSRTLSQLYCSYGPLT Predict reactive species |
| Full Name | MUS81 endonuclease homolog (S. cerevisiae) |
| Calculated Molecular Weight | 61 kDa |
| Observed Molecular Weight | 52-72 kDa |
| GenBank Accession Number | BC009999 |
| Gene Symbol | MUS81 |
| Gene ID (NCBI) | 80198 |
| RRID | AB_2147130 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96NY9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The Crossover junction endonuclease MUS81 has associated endonuclease activity against structure-specific oligonucleotide substrates, including synthetic Holliday junctions(PMID:11741546). It interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. The open reading frame of human MUS81 predicts a translation product of 551 amino acids with a molecular mass of 59 kD. There are some reports showed that the enzyme can express a 72 kDa protein in human tissues(PMID:12724407).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MUS81 antibody 11018-1-AP | Download protocol |
| WB protocol for MUS81 antibody 11018-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
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| Species | Application | Title |
|---|---|---|
Cell EMSY inhibits homologous recombination repair and the interferon response, promoting lung cancer immune evasion.
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Am J Transl Res MUS81 inhibits cell proliferation and migration in breast cancer by promoting the expression of CDKN2A(p16(INK4a)).
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Reviews
What customers say
The single review for this product is highly positive, rated 5 out of 5. The user performed a Western Blot on HeLa cells using a 1:1000 dilution and observed two bands at 70 kDa, noting that the antibody worked well at that dilution. Overall, the feedback indicates reliable performance for WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Arnaud (Verified Customer) (09-26-2025) | 2 bands at 70kDa, work well at 1:1000 dilution
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