Tested Applications
| Positive WB detected in | MCF-7 cells, A549 cells, NIH/3T3 cells, Hela cells, HEK-293 cells, HepG2 cells, L02 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67351-1-Ig targets MUS81 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag29089 Product name: Recombinant human MUS81 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 202-551 aa of BC009999 Sequence: RYSLTPEGLELAQKLAESEGLSLLNVGIGPKEPPGEETAVPGAASAELASEAGVQQQPLELRPGEYRVLLCVDIGETRGGGHRPELLRELQRLHVTHTVRKLHVGDFVWVAQETNPRDPANPGELVLDHIVERKRLDDLCSSIIDGRFREQKFRLKRCGLERRVYLVEEHGSVHNLSLPESTLLQAVTNTQVIDGFFVKRTADIKESAAYLALLTRGLQRLYQGHTLRSRPWGTPGNPESGAMTSPNPLCSLLTFSDFNAGAIKNKAQSVREVFARQLMQVRGVSGEKAAALVDRYSTPASLLAAYDACATPKEQETLLSTIKCGRLQRNLGPALSRTLSQLYCSYGPLT Predict reactive species |
| Full Name | MUS81 endonuclease homolog (S. cerevisiae) |
| Calculated Molecular Weight | 61 kDa |
| Observed Molecular Weight | 52-72 kDa |
| GenBank Accession Number | BC009999 |
| Gene Symbol | MUS81 |
| Gene ID (NCBI) | 80198 |
| RRID | AB_2918486 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q96NY9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MUS81 antibody 67351-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
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| Species | Application | Title |
|---|---|---|
Nat Commun Mitotic DNA repair by TMEJ suppresses replication stress-induced nuclear envelope reassembly defect.
| ||
J Mol Biol Mitotic DNA synthesis in untransformed human cells preserves common fragile site stability via a FANCD2-driven mechanism that requires HELQ
| ||
Front Oncol MUS81 Inhibition Enhances the Anticancer Efficacy of Talazoparib by Impairing ATR/CHK1 Signaling Pathway in Gastric Cancer.
|
Reviews
What customers say
Both reviewers rate this antibody 5/5 for Western blot against MUS81. It is described as very potent and specific, with a single band at the expected ~65–70 kDa size that disappears in siRNA-treated and conditional knockout cells, confirming specificity. One reviewer uses it at 1:10,000 in lysates and SLX4/EME1 immunoprecipitates; the other confirms strong performance in extracts and IPs up to 1:50,000.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Ivan (Verified Customer) (01-16-2026) | Really potent and specific primary antibody against MUS81. We normally use it 1:10000 for the detection of MUS81 in lysates and also in SLX4 or EME1 immunoprecipitates. Single band at expected size of 65-70 kDa that disappears in lysates from cells treated with siRNA against MUS81. Also gone in lysates from conditional MUS81 knock-out cell lines.
|
Wissem (Verified Customer) (06-19-2024) | Antibody works very well to detect MUS81 in extracts and IPs at 1:10,000 - 1:50,000
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