MUS81 Monoclonal antibody

• Featured Product
• KD/KO Validated

MUS81 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

2D9B7

Reviews (1)

Cat no : 67351-1-Ig

Synonyms

FLJ21012, FLJ44872, MUS81

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Validation Data Gallery

• 

  WB analysis using 67351-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67351-1-Ig (MUS81 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 67351-1-Ig

  WB result of MUS81 antibody (67351-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MUS81 transfected MCF-7 cells.

• 

  WB analysis of NIH/3T3 using 67351-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67351-1-Ig (MUS81 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: MCF-7 cells, A549 cells, NIH/3T3 cells, Hela cells, HEK-293 cells, HepG2 cells, L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 2 publications below

Product Information

67351-1-Ig targets MUS81 in WB, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: MUS81 fusion protein Ag29089
• Full Name: MUS81 endonuclease homolog (S. cerevisiae)
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 52-72 kDa
• GenBank Accession Number: BC009999
• Gene Symbol: MUS81
• Gene ID (NCBI): 80198
• RRID: AB_2918486
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Mol Biol; Mitotic DNA synthesis in untransformed human cells preserves common fragile site stability via a FANCD2-driven mechanism that requires HELQ; Authors - Emma L Traband ; KD Validated
human	WB	Front Oncol; MUS81 Inhibition Enhances the Anticancer Efficacy of Talazoparib by Impairing ATR/CHK1 Signaling Pathway in Gastric Cancer.; Authors - Tao Wang ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Wissem (Verified Customer) (06-19-2024)

Antibody works very well to detect MUS81 in extracts and IPs at 1:10,000 - 1:50,000

• Applications: Western Blot
• Primary Antibody Dilution: 1:10,000

MUS81 Antibody Western Blot validation (1:10,000 dilution) in (Cat no:67351-1-Ig)