Methylmalonyl Coenzyme A mutase/MUT Polyclonal antibody

Methylmalonyl Coenzyme A mutase/MUT Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 17034-1-AP

Synonyms

MUT, Methylmalonyl-CoA mutase, mitochondrial, Methylmalonyl-CoA isomerase, Methylmalonyl Coenzyme A mutase, Methylmalonyl CoA isomerase

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Validation Data Gallery

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  WB analysis using 17034-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17034-1-AP (Methylmalonyl Coenzyme A mutase/MUT antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 17034-1-AP

  IP result of anti-Methylmalonyl Coenzyme A mutase/MUT (IP:17034-1-AP, 4ug; Detection:17034-1-AP 1:500) with HepG2 cells lysate 1800ug.

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  IHC staining of human liver cancer using 17034-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17034-1-AP (Methylmalonyl Coenzyme A mutase/MUT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 17034-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17034-1-AP (Methylmalonyl Coenzyme A mutase/MUT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 17034-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17034-1-AP (Methylmalonyl Coenzyme A mutase/MUT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 17034-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17034-1-AP (Methylmalonyl Coenzyme A mutase/MUT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human liver cancer tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 3 publications below

Product Information

17034-1-AP targets Methylmalonyl Coenzyme A mutase/MUT in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Methylmalonyl Coenzyme A mutase/MUT fusion protein Ag10523
• Full Name: methylmalonyl Coenzyme A mutase
• Calculated Molecular Weight: 750 aa, 83 kDa
• Observed Molecular Weight: 78 kDa
• GenBank Accession Number: BC016282
• Gene Symbol: MUT
• Gene ID (NCBI): 4594
• RRID: AB_2147263
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IHC	Hepatology; Promoterless, Nuclease-Free Genome Editing Confers a Growth Advantage for Corrected Hepatocytes in Mice With Methylmalonic Acidemia.; Authors - Randy J Chandler
human,mouse	WB	Cells; Inactivity of Peptidase ClpP Causes Primary Accumulation of Mitochondrial Disaggregase ClpX with Its Interacting Nucleoid Proteins, and of mtDNA.; Authors - Jana Key
mouse	IHC	PLoS One; Novel AAV-mediated genome editing therapy improves health and survival in a mouse model of methylmalonic acidemia; Authors - Shengwen Zhang
mouse	WB	EMBO Mol Med; A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy; Authors - Nneka Southwell
human	WB	Biochim Biophys Acta Mol Basis Dis; ACSS3 regulates the metabolic homeostasis of epithelial cells and alleviates pulmonary fibrosis; Authors - Lan Wang
human	WB,IHC	Am J Pathol; Aging-associated Metabolite Methylmalonic Acid Increases Susceptibility to Pulmonary Fibrosis; Authors - Kai Xu ; KD Validated