MUTED/BLOC1S5 Polyclonal antibody

• Featured Product
• KD/KO Validated

MUTED/BLOC1S5 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 24015-1-AP

Synonyms

BL1S5, BLOC1S5, MU, MUTED, muted homolog (mouse), Protein Muted homolog

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Validation Data Gallery

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  WB analysis of HEK-293T using 24015-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 24015-1-AP (MUTED Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 24015-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 24015-1-AP (MUTED antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293T cells
• Positive IF detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

The immunogen of 24015-1-AP is MUTED/BLOC1S5 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MUTED/BLOC1S5 fusion protein Ag21226
• Full Name: muted homolog (mouse)
• Calculated Molecular Weight: 187 aa, 22 kDa
• Observed Molecular Weight: 22-25 kDa
• GenBank Accession Number: BC119644
• Gene Symbol: MUTED
• Gene ID (NCBI): 63915
• RRID: AB_2879402
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BLOC1S5 (biogenesis of lysosomal organelles complex 1 subunit 5) encodes a subunit of the BLOC1, a complex that is involved in transport of some but not all cargo to the lysosome and lysosome-related organelles. BLOC1S5 has a crucial role in STING-mediated cell death upon stimulation with low concentrations of CMA(PMID: 29033128).

Publications

Species	Application	Title
human	WB	Cell; The DNA Inflammasome in Human Myeloid Cells Is Initiated by a STING-Cell Death Program Upstream of NLRP3.; Authors - Moritz M Gaidt ; KO Validated
mouse	WB	Cells; Dysbindin deficiency Alters Cardiac BLOC-1 Complex and Myozap Levels in Mice.; Authors - Ankush Borlepawar