MUTYH Polyclonal antibody

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  WB analysis of K-562 using 15237-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15237-1-AP (MUTYH antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 15237-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 15237-1-AP (MUTYH antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15237-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15237-1-AP (MUTYH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

MUTYH Polyclonal Antibody for WB, ELISA

Cat No. 15237-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

hMYH, MutY homolog, mutY homolog (E. coli), MUTYH, MYH

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Tested Applications

• Positive WB detected in: K-562 cells, HeLa cells, Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15237-1-AP targets MUTYH in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7359

  Product name: Recombinant human MUTYH protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 187-535 aa of BC003178

  Sequence: MPRTAETLQQLLPGVGRYTAGAIASIAFGQATGVVDGNVARVLCRVRAIGADPSSTLVSQQLWGLAQQLVDPARPGDFNQAAMELGATVCTPQRPLCSQCPVESLCRARQRVEQEQLLASGSLSGSPDVEECAPNTGQCHLCLPPSEPWDQTLGVVNFPRKASRKPPREESSATCVLEQPGALGAQILLVQRPNSGLLAGLWEFPSVTWEPSEQLQRKALLQELQRWAGPLPATHLRHLGEVVHTFSHIKLTYQVYGLALEGQTPVTTVPPGARWLTQEEFHTAAVSTAMKKVFRVYQGQQPGTCMGSKRSQVSSPCSRKKPRMGQQVLDNFFRSHISTDAHSLNSAAQ

• Full Name: mutY homolog (E. coli)
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 53 kDa, 65 kDa
• GenBank Accession Number: BC003178
• Gene Symbol: MUTYH
• Gene ID (NCBI): 4595
• RRID: AB_2147408
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UIF7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MUTYH(MutY homolog) is also named as MYH and belongs to the Nth/MutY family. The gene enconde an A/G-specific adenine DNA glycosylase that it is involved in oxidative DNA damage repair and initiates repair of A*oxoG to C*G by removing the inappropriately paired adenine base from the DNA backbone. Possesses both adenine and 2-OH-A DNA glycosylase activities(PMID:10684930). Defects in MUTYH are a cause of familial adenomatous polyposis type 2 (FAP2) and gastric cancer (GASC). It has 6 isoforms produced by alternative splicing with the molecular weight between 57 kDa and 60 kDa.