MUTYH Polyclonal antibody

MUTYH Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 15237-1-AP

Synonyms

hMYH, MutY homolog, mutY homolog (E. coli), MUTYH, MYH



Tested Applications

Positive WB detected inK-562 cells, HeLa cells, Raji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15237-1-AP targets MUTYH in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MUTYH fusion protein Ag7359
Full Name mutY homolog (E. coli)
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 53 kDa, 65 kDa
GenBank Accession NumberBC003178
Gene Symbol MUTYH
Gene ID (NCBI) 4595
RRIDAB_2147408
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MUTYH(MutY homolog) is also named as MYH and belongs to the Nth/MutY family. The gene enconde an A/G-specific adenine DNA glycosylase that it is involved in oxidative DNA damage repair and initiates repair of A*oxoG to C*G by removing the inappropriately paired adenine base from the DNA backbone. Possesses both adenine and 2-OH-A DNA glycosylase activities(PMID:10684930). Defects in MUTYH are a cause of familial adenomatous polyposis type 2 (FAP2) and gastric cancer (GASC). It has 6 isoforms produced by alternative splicing with the molecular weight between 57 kDa and 60 kDa.

Protocols

Product Specific Protocols
WB protocol for MUTYH antibody 15237-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols