Tested Applications
| Positive WB detected in | HCT 116 cells, rat liver tissue, HepG2 cells, K-562 cells |
| Positive IHC detected in | human colon tissue, human lung cancer tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells, A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15331-1-AP targets MVD in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7255 Product name: Recombinant human MVD protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-400 aa of BC000011 Sequence: MASEKPLAAVTCTAPVNIAVIKYWGKRDEELVLPINSSLSVTLHQDQLKTTTTAVISKDFTEDRIWLNGREEDVGQPRLQACLREIRCLARKRRNSRDGDPLPSSLSCKVHVASVNNFPTAAGLASSAAGYACLAYTLARVYGVESDLSEVARRGSGSACRSLYGGFVEWQMGEQADGKDSIARQVAPESHWPELRVLILVVSAEKKLTGSTVGMRASVETSPLLRFRAESVVPARMAEMARCIRERDFPSFAQLTMKDSNQFHATCLDTFPPISYLNAISWRIIHLVHRFNAHHGDTKVAYTFDAGPNAVIFTLDDTVAEFVAAVWHGFPPGSNGDTFLKGLQVRPAPLSAELQAALAMEPTPGGVKYIIVTQVGPGPQILDDPCAHLLGPDGLPKPAA Predict reactive species |
| Full Name | mevalonate (diphospho) decarboxylase |
| Calculated Molecular Weight | 43 kDa |
| Observed Molecular Weight | 66-74 kDa, 45 kDa, 37 kDa |
| GenBank Accession Number | BC000011 |
| Gene Symbol | MVD |
| Gene ID (NCBI) | 4597 |
| RRID | AB_2147439 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P53602 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The enzyme mevalonate pyrophosphate decarboxylase(MVD) catalyzes the conversion of mevalonate pyrophosphate into isopentenyl pyrophosphate. It is also named as MPD and as a unique enzyme in one of the early steps in cholesterol biosynthesis, MVD may be a useful target for drugs aimed at lowering serum cholesterol levels(PMID:8626466). The intracellular glycosylation does not contribute to the difference between the 45 and 37 kDa species of MVD. The native MVD has a molecular weight of 90 kDa that it consists of two identical subunits of 45 kDa and a 37 kDa protein is also found as a subunit of MVD and this type of MVD may be a 74 kDa.But the 37 kDa enzyme appeared only when the rats are fed the CP diet.(PMID:9348097).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MVD antibody 15331-1-AP | Download protocol |
| IHC protocol for MVD antibody 15331-1-AP | Download protocol |
| WB protocol for MVD antibody 15331-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MVD Polyclonal antibody (catalog number 15331-1-AP) has 10 total citations. These appear in journals including Cancer Cell, J Nanobiotechnology, Phytomedicine, Mater Today Bio, Front Pharmacol, Sci Rep, Cell Signal, Curr Issues Mol Biol, J Hepatocell Carcinoma, and Mol Cell Endocrinol. The associated research fields span cancer biology (pyroptosis, thyroid cancer, hepatocellular carcinoma, T-ALL), lipid and cholesterol metabolism, mevalonate pathway signaling, endometrial regeneration, gut microbiome, and steroid biosynthesis. Applications include WB, IF, and IHC across human, mouse, and rat species.
| Species | Application | Title |
|---|---|---|
Cancer Cell Targeting the mevalonate pathway suppresses ARID1A-inactivated cancers by promoting pyroptosis | ||
J Nanobiotechnology Piezo-herbal microneedle patches enable wireless endometrial regeneration and fertility recovery. | ||
Phytomedicine Xia Ku Cao Paste restores intestinal microbiota homeostasis and improves hepatic metabolism disturbances to alleviate hyperlipidemia. | ||
Mater Today Bio Spatiotemporal piezoelectric microspheres for wireless endometrial repair with improved pregnancy outcomes. | ||
Front Pharmacol CCR9 overexpression promotes T-ALL progression by enhancing cholesterol biosynthesis | ||
Sci Rep Effects of Paeoniae Radix Rubra on lowering lipid via bioinformatics and gut microbiome. |
Reviews
What customers say
The single review for this product rates it 4 out of 5 stars for Western Blot at a 1:1000 dilution. The reviewer reports consistently obtaining good bands, indicating reliable performance and reproducibility in their WB experiments.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
k (Verified Customer) (06-10-2021) | We always got good bands in western with this Ab
|
