MVK Polyclonal antibody

MVK Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 12228-1-AP

Synonyms

MVLK, MK, Mevalonate kinase, LRBP, EC:2.7.1.36

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Validation Data Gallery

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  WB analysis using 12228-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12228-1-AP (MVK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 12228-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12228-1-AP (MVK antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse uterus using 12228-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 12228-1-AP (MVK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 12228-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12228-1-AP (MVK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse uterus using 12228-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 12228-1-AP (MVK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of PC-3 using 12228-1-AP

  Immunofluorescent analysis of PC-3 cells using 12228-1-AP (MVK antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Caco-2 cells, PC-3 cells, mouse uterus tissue, Jurkat cells, HepG2 cells
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below

Product Information

12228-1-AP targets MVK in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MVK fusion protein Ag2865
• Full Name: mevalonate kinase
• Calculated Molecular Weight: 396 aa, 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC016140
• Gene Symbol: MVK
• Gene ID (NCBI): 4598
• RRID: AB_2147565
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MVK (Mevalonate kinase) is a 42 kDa cytoplasmic protein that belongs to the GHMP kinase family. Mevalonate kinase catalyzes the ATP-dependent phosphorylation of mevalonic acid to form mevalonate 5-phosphate. Defects in mevalonate kinase can cause mevalonic aciduria (MEVA). It is an accumulation of mevalonic acid which causes a variety of symptoms such as psychomotor retardation, dysmorphic features, cataracts, hepatosplenomegaly, lymphadenopathy, anemia, hypotonia, myopathy and ataxia.

Publications

Species	Application	Title
mouse	WB	Bioorg Chem; Gypenoside L inhibits hepatocellular carcinoma by targeting the SREBP2-HMGCS1 axis and enhancing immune response; Authors - Man-Yu Xiao
human	WB	J Hepatocell Carcinoma; miR-612 Enhances RSL3-Induced Ferroptosis of Hepatocellular Carcinoma Cells via Mevalonate Pathway; Authors - Kailin Xing
mouse	WB	Curr Issues Mol Biol; Gigantol Improves Cholesterol Metabolism and Progesterone Biosynthesis in MA-10 Leydig Cells.; Authors - Audrey Basque
human	WB	Exp Ther Med; Effects of mevalonate kinase interference on cell differentiation, apoptosis, prenylation and geranylgeranylation of human keratinocytes are attenuated by farnesyl pyrophosphate or geranylgeranyl pyrophosphate.; Authors - Min Li
mouse	WB	Toxicol In Vitro; Sinapic and ferulic acid phenethyl esters increase the expression of steroidogenic genes in MA-10 tumor Leydig cells; Authors - Audrey Basque