Tested Applications
| Positive WB detected in | Caco-2 cells, PC-3 cells, mouse uterus tissue, Jurkat cells, HepG2 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12228-1-AP targets MVK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2865 Product name: Recombinant human MVK protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-396 aa of BC016140 Sequence: MLSEVLLVSAPGKVILHGEHAVVHGKVALAVSLNLRTFLRLQPHSNGKVDLSLPNIGIKRAWDVARLQSLDTSFLEQGDVTTPTSEQVEKLKEVAGLPDDCAVTERLAVLAFLYLYLSICRKQRALPSLDIVVWSELPPGAGLGSSAAYSVCLAAALLTVCEEIPNPLKDGDCVNRWTKEDLELINKWAFQGERMIHGNPSGVDNAVSTWGGALRYHQGKISSLKRSPALQILLTNTKVPRNTRALVAGVRNRLLKFPEIVAPLLTSIDAISLECERVLGEMGEAPAPEQYLVLEELIDMNQHHLNALGVGHASLDQLCQVTRARGLHSKLTGAGGGGCGITLLKPGLEQPEVEATKQALTSCGFDCLETSIGAPGVSIHSATSLDSRVQQALDGL Predict reactive species |
| Full Name | mevalonate kinase |
| Calculated Molecular Weight | 396 aa, 42 kDa |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | BC016140 |
| Gene Symbol | MVK |
| Gene ID (NCBI) | 4598 |
| RRID | AB_2147565 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q03426 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MVK (Mevalonate kinase) is a 42 kDa cytoplasmic protein that belongs to the GHMP kinase family. Mevalonate kinase catalyzes the ATP-dependent phosphorylation of mevalonic acid to form mevalonate 5-phosphate. Defects in mevalonate kinase can cause mevalonic aciduria (MEVA). It is an accumulation of mevalonic acid which causes a variety of symptoms such as psychomotor retardation, dysmorphic features, cataracts, hepatosplenomegaly, lymphadenopathy, anemia, hypotonia, myopathy and ataxia.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MVK antibody 12228-1-AP | Download protocol |
| IHC protocol for MVK antibody 12228-1-AP | Download protocol |
| WB protocol for MVK antibody 12228-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-MVK polyclonal antibody (Cat# 12228-1-AP) has 13 total citations. These appear in journals including Nature Cancer, Nature Aging, Journal of Ethnopharmacology, Bioorganic Chemistry, Scientific Reports, European Journal of Medical Research, Current Issues in Molecular Biology, Analytical and Bioanalytical Chemistry, Journal of Hepatocellular Carcinoma, Molecular and Cellular Endocrinology, Toxicology in Vitro, Animal Reproduction Science, and Experimental & Therapeutic Medicine. Research fields cover lipid/cholesterol metabolism, cancer biology, reproductive endocrinology, and ferroptosis.
| Species | Application | Title |
|---|---|---|
Nat Cancer CHD1 loss reprograms SREBP2-driven cholesterol synthesis to fuel androgen-responsive growth and castration resistance in SPOP-mutated prostate tumors | ||
Nat Aging Mevalonate metabolites boost aged oocyte quality through prenylation of small GTPases. | ||
J Ethnopharmacol Tylophora yunnanensis extract inhibits cholesterol biosynthesis to suppress triple negative breast cancer | ||
Bioorg Chem Gypenoside L inhibits hepatocellular carcinoma by targeting the SREBP2-HMGCS1 axis and enhancing immune response | ||
Sci Rep Effects of Paeoniae Radix Rubra on lowering lipid via bioinformatics and gut microbiome. | ||
Eur J Med Res MVK, induced by Kras, represses cGAS-Sting signalling in lung adenocarcinoma |
