Tested Applications
| Positive WB detected in | A549 cells, RAW264.7, human brain tissue, mouse lung tissue, rat lung tissue, SH-SY5Y cells |
| Positive IP detected in | mouse lung tissue |
| Positive IHC detected in | human breast cancer tissue, human testis tissue, human skin tissue, human lung cancer tissue, human kidney tissue, human normal colon Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16478-1-AP targets MVP/LRP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9593 Product name: Recombinant human MVP protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 544-893 aa of BC015623 Sequence: NWHFEVNDRKDPQETAKLFSVPDFVGDACKAIASRVRGAVASVTFDDFHKNSARIIRTAVFGFETSEAKGPDGMALPRPRDQAVFPQNGLVVSSVDVQSVEPVDQRTRDALQRSVQLAIEITTNSQEAAAKHEAQRLEQEARGRLERQKILDQSEAEKARKELLELEALSMAVESTGTAKAEAESRAEAARIEGEGSVLQAKLKAQALAIETEAELQRVQKVRELELVYARAQLELEVSKAQQLAEVEVKKFKQMTEAIGPSTIRDLAVAGPEMQVKLLQSLGLKSTLITDGSTPINLFNTAFGLLGMGPEGQPLGRRVASGPSPGEGISPQSAQAPQAPGDNHVVPVLR Predict reactive species |
| Full Name | major vault protein |
| Calculated Molecular Weight | 893 aa, 99 kDa |
| Observed Molecular Weight | 99-104 kDa |
| GenBank Accession Number | BC015623 |
| Gene Symbol | LRP |
| Gene ID (NCBI) | 9961 |
| RRID | AB_2147597 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14764 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Vaults are the largest cellular ribonuclear protein complexes with a hollow barrel-like structure, and have been associated with the MDR phenotype. Major vault protein (MVP) is the main component of vaults and is presumed to be involved in MDR. MVP is identical with the human lung resistance protein (LRP), known to be overexpressed in multiple chemotherapy resistance models [PMID:23083532]. MVP has been associated to resistance to radiotherapy [4], probably due to its role in preventing apoptosis by inhibiting the COP-1/p53 axis [PMID: 12894554, 15994960].
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MVP/LRP antibody 16478-1-AP | Download protocol |
| IHC protocol for MVP/LRP antibody 16478-1-AP | Download protocol |
| IP protocol for MVP/LRP antibody 16478-1-AP | Download protocol |
| WB protocol for MVP/LRP antibody 16478-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-Major Vault Protein (MVP) antibody (Cat# 16478-1-AP) has 24 citations across journals including Science, Cell, Nature Communications, Advanced Science, Journal of Extracellular Vesicles, Cell Reports, Frontiers in Immunology, Journal of Cell Biology, and Scientific Reports. Research fields span cancer biology (colon, lung, pancreatic, thyroid, gastric, ovarian, hepatocellular carcinoma), extracellular vesicle/exosome biology, RNA processing, drug resistance, immune regulation, ribosome function, and virology.
| Species | Application | Title |
|---|---|---|
Cell The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity. | ||
Drug Resist Updat CHD4 promotes acquired chemoresistance and tumor progression by activating the MEK/ERK axis
| ||
J Extracell Vesicles Identification of a Biomarker Panel in Extracellular Vesicles Derived From Non-Small Cell Lung Cancer (NSCLC) Through Proteomic Analysis and Machine Learning | ||
Nat Commun MVP-mediated exosomal sorting of miR-193a promotes colon cancer progression.
| ||
Adv Sci (Weinh) Tryptophan As a New Member of RNA-Induced Silencing Complexes Prevents Colon Cancer Liver Metastasis |
Reviews
What customers say
The single review for 16478-1-AP (MVP antibody) received a 5-star rating. The reviewer used it at a 1:2000 dilution for Western Blot on mouse liver tissue lysates, performing SDS-PAGE followed by overnight incubation in 1X TBST. The reviewer reported successful detection without additional comments on specificity or sensitivity. Overall, the feedback is positive, confirming reliable performance under standard WB conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Kamal (Verified Customer) (01-22-2026) | Liver lysates were subjected to SDS PAGE followed by western blot with MVP antibody at dilution of 1:2000 in 1X TBST incubated overnight.
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