MX1 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 82677-1-RR (same clone as 82677-1-PBS)

  Untreated and IFN alpha treated A549 cells, untreated and IFN alpha treated HepG2 cells were subjected to SDS PAGE followed by western blot with 82677-1-RR (MX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82677-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 82677-1-RR (same clone as 82677-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82677-1-RR (MX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82677-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 82677-1-RR (same clone as 82677-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82677-1-RR (MX1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82677-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 82677-1-RR (same clone as 82677-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82677-1-RR (MX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82677-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 82677-1-RR (same clone as 82677-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82677-1-RR (MX1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82677-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 82677-1-RR (same clone as 82677-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using MX1 antibody (82677-1-RR, Clone: 2L19 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82677-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82677-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82677-1-RR against Human MX1 were performed. The affinity constant is 0.763 nM.

MX1 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 82677-1-PBS

Clone No.2L19

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Interferon-regulated resistance GTP-binding protein MxA, Interferon-induced protein p78, Interferon-induced GTP-binding protein Mx1, N-terminally processed, Interferon-induced GTP-binding protein Mx1, IFI-78K

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Product Information

82677-1-PBS targets MX1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag4692

  Product name: Recombinant human MX1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 312-662 aa of BC032602

  Sequence: LLEEGKATVPCLAEKLTSELITHICKSLPLLENQIKETHQRITEELQKYGVDIPEDENEKMFFLIDKINAFNQDITALMQGEETVGEEDIRLFTRLRHEFHKWSTIIENNFQEGHKILSRKIQKFENQYRGRELPGFVNYRTFETIVKQQIKALEEPAVDMLHTVTDMVRLAFTDVSIKNFEEFFNLHRTAKSKIEDIRAEQEREGEKLIRLHFQMEQIVYCQDQVYRGALQKVREKELEEEKKKKSWDFGAFQSSSATDSSMEEIFQHLMAYHQEASKRISSHIPLIIQFFMLQTYGQQLQKAMLQLLQDKDTYSWLLKERSDTSDKRKFLKERLARLTQARRRLAQFPG

• Full Name: Myxovirus resistance protein 1
• Calculated Molecular Weight: 662 aa, 76 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC032602
• Gene Symbol: MX1
• Gene ID (NCBI): 4599
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P20591
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Mx1 (also known as MxA) is a GTPase that belongs to the Mx family of proteins, which are ubiquitous in eukaryotes and involved in the immune response to viral infections. The human Mx1 is localized in the cytoplasm interfering with the translation of viral proteins, whereas the mouse Mx1 accumulates in the nucleus and inhibits the primary transcription of viral RNA. Mx1 expression is induced by treatment with α/β IFN.