MX2 Polyclonal antibody

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• MX2 Polyclonal antibody (13278-1-AP)

MX2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 13278-1-AP Copied!

Publications (6)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Synonyms

p78-related protein, p78 related protein, Myxovirus resistance protein 2, MXB, Interferon-regulated resistance GTP-binding protein MxB

• 

  WB analysis using 13278-1-AP

  Untreated and IFN beta treated THP-1 cell lysates were subjected to SDS PAGE followed by western blot with 13278-1-AP (MX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach using 13278-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13278-1-AP (MX2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: THP-1 cells, IFN beta treated THP-1 cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13278-1-AP targets MX2 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4020

  Product name: Recombinant human MX2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 369-715 aa of BC035293

  Sequence: RLAERLTTELIMHIQKSLPLLEGQIRESHQKATEELRRCGADIPSQEADKMFFLIEKIKMFNQDIEKLVEGEEVVRENETRLYNKIREDFKNWVGILATNTQKVKNIIHEEVEKYEKQYRGKELLGFVNYKTFEIIVHQYIQQLVEPALSMLQKAMEIIQQAFINVAKKHFGEFFNLNQTVQSTIEDIKVKHTAKAENMIQLQFRMEQMVFCQDQIYSVVLKKVREEIFNPLGTPSQNMKLNSHFPSNESSVSSFTEIGIHLNAYFLETSKRLANQIPFIIQYFMLRENGDSLQKAMMQILQEKNRYSWLLQEQSETATKRRILKERIYRLTQARHALCQFSSKEIH

• Full Name: myxovirus (influenza virus) resistance 2 (mouse)
• Calculated Molecular Weight: 715 aa, 82 kDa
• Observed Molecular Weight: 70-82 kDa
• GenBank Accession Number: BC035293
• Gene Symbol: MX2
• Gene ID (NCBI): 4600
• RRID: AB_3669157
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P20592
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Myxovirus resistance 2 (MX2, also known as MxB) is a member of both the dynamin family and the family of large GTPases. MX2 is an interferon-stimulated gene that inhibits the nuclear import of HIV-1 and interacts with the viral capsid and cellular nuclear transport machinery. MX2 mediates the effects of IFN signaling in a cell type and context-dependent manner that can influence cells sensitivity to MAPK pathway targeted therapies. MX2 is also associated with the tumorigenesis process in melanoma(PMID: 33734579).

Publications

What published studies show

The MX2 Polyclonal antibody (Cat# 13278-1-AP) has 6 citations published in Cell, Nature Communications, Annals of Medicine, Journal of Virology (×2), and Veterinary Microbiology. Research fields span viral infection mechanisms (poxvirus, DNA virus, TGEV), mTOR signaling regulation, antiviral immune responses, interferon-stimulated gene expression, JAK-STAT1 pathway, and kidney renal clear cell carcinoma prognosis. Applications include Western blot and IHC in human and pig samples.

Automatically generated from the 6 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell; Poxviruses Evade Cytosolic Sensing through Disruption of an mTORC1-mTORC2 Regulatory Circuit.; Authors - Nathan Meade
human	WB	Nat Commun; The poxvirus F17 protein counteracts mitochondrially orchestrated antiviral responses; Authors - Nathan Meade
human	IHC	Ann Med; A novel glycogene-related signature for prognostic prediction and immune microenvironment assessment in kidney renal clear cell carcinoma; Authors - Xuyan Zhao
human	WB	J Virol; mTOR Dysregulation by Vaccinia Virus F17 Controls Multiple Processes with Varying Roles in Infection.; Authors - Nathan Meade
human	WB	J Virol; YTHDF2 Is Downregulated in Response to Host Shutoff Induced by DNA Virus Infection and Regulates Interferon-Stimulated Gene Expression; Authors - Charles R Hesser
pig	WB	Vet Microbiol; Ursodeoxycholic acid against TGEV infection via the JAK-STAT1 signaling pathway.; Authors - Zhiwei Sun