MXD1 Polyclonal antibody

• Home
• Products
• Primary Antibodies
• MXD1 Polyclonal antibody (19547-1-AP)

MXD1 Polyclonal Antibody for WB, IP, ELISA

Cat No. 19547-1-AP Copied!

Publications (3)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, chIP, ELISA

Synonyms

MAD, MAD1, MAX dimerization protein 1, Max dimerizer 1, Protein MAD

• 

  WB analysis of HepG2 using 19547-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19547-1-AP (MXD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HepG2 using 19547-1-AP

  IP result of anti-MXD1 (IP:19547-1-AP, 4ug; Detection:19547-1-AP 1:800) with HepG2 cells lysate 2000 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for MXD1 in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: HepG2 cells
• Positive IP detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19547-1-AP targets MXD1 in WB, IP, chIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: MAX dimerization protein 1
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: NM_002357
• Gene Symbol: MXD1
• Gene ID (NCBI): 4084
• RRID: AB_10641995
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q05195
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MXD1, also named as MAD, is a transcriptional repressor. MAD1 binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. MAD thus antagonizes MYC transcriptional activity by competing for MAX. There're two isoforms of MXD1 protein.

Publications

What published studies show

MXD1 Polyclonal antibody (Cat# 19547-1-AP) has 3 total citations published in Theranostics, Journal of Biological Chemistry, and Cell Reports. The associated research fields include m6A RNA modification and retinal neovascularization, glucose metabolism and long noncoding RNA regulation, and CRAC channel signaling in autoimmune inflammation.

Automatically generated from the 3 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,ChIP	Theranostics; BARD1-mediated stabilization of METTL14 promotes retinal neovascularization by m6A-modifying MXD1 mRNA on a YTHDF2-dependent manner; Authors - Xianyang Liu
human	chIP	J Biol Chem; Role for carbohydrate response element-binding protein (ChREBP) in high glucose-mediated repression of long noncoding RNA Tug1.; Authors - Jianyin Long
mouse, human		Cell Rep; Temporal CRAC channel blockade modulates the MYC-MXD axis to attenuate autoimmune inflammation.; Authors - Beibei Wu