MYBBP1A Polyclonal antibody

• Featured Product
• KD/KO Validated

MYBBP1A Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 14524-1-AP

Synonyms

MYB binding protein (P160) 1a, Myb binding protein 1A, MYBBP1A, P160, PAP2

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Validation Data Gallery

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  WB analysis using 14524-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14524-1-AP (MYBBP1A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14524-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14524-1-AP (MYBBP1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 14524-1-AP

  IP result of anti-MYBBP1A (IP:14524-1-AP, 4ug; Detection:14524-1-AP 1:500) with HEK-293 cells lysate 2800ug.

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  IHC staining of human kidney using 14524-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14524-1-AP (MYBBP1A antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 14524-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14524-1-AP (MYBBP1A antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HepG2 using 14524-1-AP

  Immunofluorescent analysis of HepG2 cells, using MYBBP1A antibody 14524-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  FC experiment of HepG2 using 14524-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug MYBBP1A antibody (14524-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Product Information

14524-1-AP targets MYBBP1A in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MYBBP1A fusion protein Ag6008
• Full Name: MYB binding protein (P160) 1a
• Calculated Molecular Weight: 149 kDa
• Observed Molecular Weight: 150-160 kDa
• GenBank Accession Number: BC050546
• Gene Symbol: MYBBP1A
• Gene ID (NCBI): 10514
• RRID: AB_2148137
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The protooncogene MYB is predominantly expressed in immature hemopoietic cells where it has an essential role in hemopoietic cell proliferation and differentiation. Oncogenically activated forms of MYB is generally N- and/or C-terminal truncations of the normal MYB protein. Removal of the C terminus of MYB disrupts or deletes a region termed the negative regulatory domain (NRD), resulting in an increase in DNA binding, transactivation, and transformation by MYB. One feature of the NRD is a leucine zipper-like motif [PMID: 8302594]. Murine Myb-binding protein-1a (MYBBP1A), originally called P160, was identified by its ability to interact specifically with Myb via this leucine zipper-like motif. MYBBP1A modulates MYB activity upon binding to the MYB NRD [PMID: 10644447, 9447996].

Publications

Species	Application	Title
mouse	WB	Cell Rep; A fetal tumor suppressor axis abrogates MLL-fusion-driven acute myeloid leukemia; Authors - Mohamed Eldeeb
mouse	WB	Sci Adv; In vivo protein turnover rates in varying oxygen tensions nominate MYBBP1A as a mediator of the hyperoxia response; Authors - Xuewen Chen
human	WB,IF	Cancers (Basel); Loss of MYBBP1A Induces Cancer Stem Cell Activity in Renal Cancer.; Authors - Blanca Felipe-Abrio ; KD Validated
human	WB	Mol Oncol; c-MYB- and PGC1a-dependent metabolic switch induced by MYBBP1A loss in renal cancer.; Authors - Blanca Felipe-Abrio