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KD/KO Validated

B-Myb Polyclonal antibody

B-Myb Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 18896-1-AP

Publications(30)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, IP, CoIP, ChIP, ELISA

Synonyms

B MYB, BMYB, B-Myb, Myb like protein 2, Myb related protein B, MYBL2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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WB analysis of HeLa using 18896-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 18896-1-AP (B-Myb antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 18896-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 18896-1-AP (B-Myb antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of HeLa using 18896-1-AP
IP result of anti-B-Myb (IP:18896-1-AP, 4ug; Detection:18896-1-AP 1:300) with HeLa cells lysate 1520ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human prostate cancer using 18896-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 18896-1-AP (B-Myb antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human prostate cancer using 18896-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 18896-1-AP (B-Myb antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (30)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human prostate cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18896-1-AP targets B-Myb in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag13525 Product name: Recombinant human MYBL2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 401-700 aa of BC007585 Sequence: SGIGTPPSVLKRQRKRRVALSPVTENSTSLSFLDSCNSLTPKSTPVKTLPFSPSQFLNFWNKQDTLELESPSLTSTPVCSQKVVVTTPLHRDKTPLHQKHAAFVTPDQKYSMDNTPHTPTPFKNALEKYGPLKPLPQTPHLEEDLKEVLRSEAGIELIIEDDIRPEKQKRKPGLRRSPIKKVRKSLALDIVDEDVKLMMSTLPKSLSLPTTAPSNSSSLTLSGIKEDNSLLNQGFLQAKPEKAAVAQKPRSHFTTPAPMSSAWKTVACGGTRDQLFMQEKARQLLGRLKPSHTSRTLILS Predict reactive species
Full Name	v-myb myeloblastosis viral oncogene homolog (avian)-like 2
Calculated Molecular Weight	79 kDa
Observed Molecular Weight	90 kDa
GenBank Accession Number	BC007585
Gene Symbol	B Myb
Gene ID (NCBI)	4605
RRID	AB_10644283
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P10244
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYBL2, also named as BMYB, is a 700 amino acid protein, which is phosphorylated by cyclin A/CDK2 during S-phase. MYBL2 is a transcription factor involved in the regulation of cell survival, proliferation, and differentiation. The calculated molecular weight of MYBL2 is 79 kDa, but modified MYBL2 is about 90 kDa (PMID: 11418655).

Protocols

Product Specific Protocols
IHC protocol for B-Myb antibody 18896-1-AP	Download protocol
IP protocol for B-Myb antibody 18896-1-AP	Download protocol
WB protocol for B-Myb antibody 18896-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
human	IHC	Cell Rep Med Distinctive multicellular immunosuppressive hubs confer different intervention strategies for left- and right-sided colon cancers Authors - Bingxin Liu View Article
human	WB	Oxid Med Cell Longev LncRNA-MALAT1 Regulates Cancer Glucose Metabolism in Prostate Cancer via MYBL2/mTOR Axis. Authors - Xiangyu Mu View Article
human,mouse	WB	J Virol Minute Virus of Mice (MVM) inhibits transcription of the cyclin B1 gene during infection. Authors - Matthew S Fuller View Article
human	WB,IHC,IP	Cancer Gene Ther A targetable MYBL2-ATAD2 axis governs cell proliferation in ovarian cancer Authors - Qun Liu KO Validated View Article
mouse	WB	Mol Cells Sertad1 Induces Neurological Injury after Ischemic Stroke via the CDK4/p-Rb Pathway. Authors - Jianxiong Li View Article
human	WB	Mediators Inflamm Knockdown MTDH Inhibits Glioma Proliferation and Migration and Promotes Apoptosis by Downregulating MYBL2 Authors - Junqi Fu View Article