B-Myb Polyclonal antibody

• Featured Product
• KD/KO Validated

B-Myb Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

17

Cat no : 18896-1-AP

Synonyms

B MYB, BMYB, B-Myb, Myb like protein 2, Myb related protein B, MYBL2

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Validation Data Gallery

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  WB analysis of HeLa using 18896-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18896-1-AP (B-Myb antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18896-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18896-1-AP (B-Myb antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 18896-1-AP

  IP result of anti-B-Myb (IP:18896-1-AP, 4ug; Detection:18896-1-AP 1:300) with HeLa cells lysate 1520ug.

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  IHC staining of human prostate cancer using 18896-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 18896-1-AP (B-Myb antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 18896-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 18896-1-AP (B-Myb antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 15 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• ChIP: See 3 publications below

Product Information

18896-1-AP targets B-Myb in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: B-Myb fusion protein Ag13525
• Full Name: v-myb myeloblastosis viral oncogene homolog (avian)-like 2
• Calculated Molecular Weight: 79 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC007585
• Gene Symbol: B Myb
• Gene ID (NCBI): 4605
• RRID: AB_10644283
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYBL2, also named as BMYB, is a 700 amino acid protein, which is phosphorylated by cyclin A/CDK2 during S-phase. MYBL2 is a transcription factor involved in the regulation of cell survival, proliferation, and differentiation. The calculated molecular weight of MYBL2 is 79 kDa, but modified MYBL2 is about 90 kDa (PMID: 11418655).

Publications

Species	Application	Title
human	IHC	Cell Rep Med; Distinctive multicellular immunosuppressive hubs confer different intervention strategies for left- and right-sided colon cancers; Authors - Bingxin Liu
human	WB	Oxid Med Cell Longev; LncRNA-MALAT1 Regulates Cancer Glucose Metabolism in Prostate Cancer via MYBL2/mTOR Axis.; Authors - Xiangyu Mu
human,mouse	WB	J Virol; Minute Virus of Mice (MVM) inhibits transcription of the cyclin B1 gene during infection.; Authors - Matthew S Fuller
human	WB,IHC,IP	Cancer Gene Ther; A targetable MYBL2-ATAD2 axis governs cell proliferation in ovarian cancer; Authors - Qun Liu ; KO Validated
mouse	WB	Mol Cells; Sertad1 Induces Neurological Injury after Ischemic Stroke via the CDK4/p-Rb Pathway.; Authors - Jianxiong Li
human	WB	Mediators Inflamm; Knockdown MTDH Inhibits Glioma Proliferation and Migration and Promotes Apoptosis by Downregulating MYBL2; Authors - Junqi Fu