MYBPC3 Polyclonal antibody

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• MYBPC3 Polyclonal antibody (19977-1-AP)

MYBPC3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 19977-1-AP Copied!

Publications (6)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Cardiac MyBP C, Cardiac MyBP-C, C-protein, cardiac muscle isoform, MYBPC 3, Myosin-binding protein C, cardiac-type

• 

  WB analysis using 19977-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19977-1-AP (MYBPC3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human heart using 19977-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 19977-1-AP (MYBPC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat heart using 19977-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 19977-1-AP (MYBPC3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse heart using 19977-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 19977-1-AP (MYBPC3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse heart using 19977-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 19977-1-AP (MYBPC3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse heart tissue, rat heart tissue, human heart tissue
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19977-1-AP targets MYBPC3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: myosin binding protein C, cardiac
• Calculated Molecular Weight: 141 kDa
• Observed Molecular Weight: 141-150 kDa
• GenBank Accession Number: NM_000256
• Gene Symbol: MYBPC3
• Gene ID (NCBI): 4607
• RRID: AB_10858232
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14896
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYBPC3 belongs to the immunoglobulin superfamily and MyBP family. MYBPC3 located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role. Defects in MYBPC3 are the cause of cardiomyopathy familial hypertrophic type 4 (CMH4). The antibody detects the full length form and the degradation bands around 28-54 kDa in SDS-PAGE.(PMID: 22076249)

Publications

What published studies show

MYBPC3 Antibody (Cat# 19977-1-AP) has 6 total citations published in J Mol Cell Cardiol, FASEB J, J Biol Chem, Front Cardiovasc Med (×2), and PLoS One. The associated research fields include cardiovascular disease, hypertrophic cardiomyopathy, heart failure, cardiac molecular biology, and sudden cardiac death. Applications cited include WB and IHC in mouse and human samples.

Automatically generated from the 6 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	J Mol Cell Cardiol; Catecholaminergic stress results in signs of heart failure in PP2A-PR72 overexpressor mice.; Authors - Paul Pauls
mouse	WB	FASEB J; Amplified pathogenic actions of angiotensin II in cysteine-rich LIM-only protein 4-negative mouse hearts.; Authors - Julia Straubinger
		J Biol Chem; Proximity-dependent proteomics and network analysis of adenylyl cyclase isoforms 5, 6, and 9 in cardiomyocytes; Authors - Taeyeop Park
human	IHC	Front Cardiovasc Med; Case Report: Identification of the First Synonymous Variant of Myosin Binding Protein C3 (c.24A>C, p.P8P) Altering RNA Splicing in a Cardiomyopathy and Sudden Cardiac Death Case.; Authors - Jie-Yuan Jin
mouse	WB	Front Cardiovasc Med; Hypercontractile cardiac phenotype in mice overexpressing the regulatory subunit PR72 of protein phosphatase 2A; Authors - Julius R Herting
human	WB	PLoS One; A novel variant in MYBPC3 causes hypertrophic cardiomyopathy by haploinsufficiency.; Authors - Yuanyuan Zhang