MYC Monoclonal antibody, PBS Only (Detector)

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  Sandwich ELISA standard curve of MP50347-2

  Sandwich ELISA standard curve of MP50347-2, Human c-MYC Monoclonal Matched Antibody Pair - PBS only. 67447-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag17519. 67447-5-PBS was HRP conjugated as the detection antibody. Range: 0.781-50 ng/mL.

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  Sample test of MP50347-2

  The mean c-MYC concentration was determined to be 10.0 ng/mL in A431 cell extract based on a 1.5 mg/mL extract load and 7.6 ng/mL in HeLa cell extract based on a 1.5 mg/mL extract load.

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  Cytometric bead array standard curve of MP50347-2

  Cytometric bead array standard curve of MP50347-2, MYC Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67447-4-PBS. Detection antibody: 67447-5-PBS. Standard:Ag17519. Range: 0.781-50 ng/mL.

MYC Monoclonal Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 67447-5-PBS

Clone No.2B5C5

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

MRTL, c-Myc, Class E basic helix-loop-helix protein 39, c Myc, bHLHe39

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Product Information

67447-5-PBS targets MYC as part of a matched antibody pair:

MP50347-2: 67447-4-PBS capture and 67447-5-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17519

  Product name: Recombinant human MYC protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 16-454 aa of BC000141

  Sequence: MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPNPARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDSLLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGSPSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNRKCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKATAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA

• Full Name: v-myc myelocytomatosis viral oncogene homolog (avian)
• Calculated Molecular Weight: 49 kDa
• GenBank Accession Number: BC000141
• Gene Symbol: MYC
• Gene ID (NCBI): 4609
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P01106
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.