Tested Applications
| Positive WB detected in | HEK-293 cells, A549 cells, HL-60 cells, HeLa cells, K-562 cells |
| Positive IHC detected in | human colon cancer tissue, mouse embryo tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, HEK-293 cells, U2OS cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
80845-1-RR targets c-MYC in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | c-MYC fusion protein Ag1263 Predict reactive species |
| Full Name | v-myc myelocytomatosis viral oncogene homolog (avian) |
| Calculated Molecular Weight | 49 kDa |
| Observed Molecular Weight | 50-65 kDa |
| GenBank Accession Number | BC000141 |
| Gene Symbol | MYC |
| Gene ID (NCBI) | 4609 |
| RRID | AB_2918915 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P01106 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MYC contains one basic helix-loop-helix (bHLH) domain. This protein is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. It seems to activate the transcription of growth-related genes. It binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for c-MYC antibody 80845-1-RR | Download protocol |
| IHC protocol for c-MYC antibody 80845-1-RR | Download protocol |
| IF protocol for c-MYC antibody 80845-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Nikolett (Verified Customer) (07-29-2025) | Antibody works well for western blot in 1:1000 dilution, incubated overnight in 3%BSA/PBS on human lung cancer cell lines and mouse lung cancer cell lines. For IHC in a 1:300 dilution upon pH6 NaCi based antigene retrieval it gives a specific nuclear signal in human lung cancer patient derived samples as well as on murine lung cancer tissue.
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