MYC tag Recombinant antibody, PBS Only

MYC tag Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 80469-2-PBS
Clone No.241611D1

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein

Applications

WB, IF/ICC, FC (Intra), IP, Indirect ELISA

241611D1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Product Information

80469-2-PBS targets MYC tag in WB, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MYC tag fusion protein Ag9409 Predict reactive species
Full Name Myc tag
Gene Symbol Myc tag
Gene ID (NCBI) 99
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The myc-tag is a short synthetic polypeptide sequence derived from c-MYC protein that can be added to recombinant proteins to enable isolation and study when an antibody is not available. This antibody recognizes the MYC tag EQKLISEEDL. MYC tag is a part of c-MYC protein. So there's weaker 62-65 kDa bands for endogenous c-MYC protein. What is the molecular weight of myc? 1203Da: the ten amino acid myc tag sequence is EQKLISEEDL (in single letter code). What are the applications for myc tag? The addition of the myc tag to a particular protein can be useful when an antibody is not available to the protein of interest. Using recombinant DNA technology, the myc tag can be fused to the protein and then an antibody against the myc tag can be used to probe (PMID: 24490106). This is a reliable method and can be used in a number of different techniques, including purification using chromatography, tracking the protein in localization studies using immunofluorescence, or quantifying levels using Western Blot (PMID: 24490106). What is the structure of myc tag? The c-myc gene from which this tag is derived has a molecular weight of 49kDa, but the myc tag represents only a small portion of the C-terminus of this gene. The short polypeptide sequence can be fused to the N-terminus or the C-terminus of any protein without influencing function, although it is advised to avoid fusing it to a secretory signal. A cleavage site behind this tag is also sometimes engineered to allow removal with a specific protease.

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