Tested Applications
| Positive WB detected in | NCI-H1299 cells, U266 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14584-1-AP targets L-Myc in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6105 Product name: Recombinant human MYCL1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-206 aa of BC011864 Sequence: MDYDSYQHYFYDYDCGEDFYRSTAPSEDIWKKFELVPSPPTSPPWGLGPGAGDPAPGIGPPEPWPGGCTGDEAESRGHSKGWGRNYASIIRRDCMWSGFSARERLERAVSDRLAPGAPRGNPPKASAAPDCTPSLEAGNPAPAAPCPLGEPKTQACSGSESPSDSGKDLPEPSKRGPPHGWPKLCPCLRSGIGSSQALGPSPPLFG Predict reactive species |
| Full Name | v-myc myelocytomatosis viral oncogene homolog 1, lung carcinoma derived (avian) |
| Calculated Molecular Weight | 40 kDa |
| Observed Molecular Weight | 68 kDa |
| GenBank Accession Number | BC011864 |
| Gene Symbol | MYCL1 |
| Gene ID (NCBI) | 4610 |
| RRID | AB_3669190 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P12524 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The MYC gene family consists of 3 members, C-MYC, L-MYC, and N-MYC, all of which belong to the superfamily of basic helix-loop-helix leucine zipper (bHLHLZ) DNA binding proteins. L-MYC encodes a nuclear phosphoprotein with 364 amino acids, heterodimerizing with partner protein Max and acts as a transcription factor to control the expression of target genes. L-Myc is involved in many biological processes, including cell proliferation, differentiation, apoptosis and has been found to be amplified and over-expressed in some malignancies. L-Myc amplification has been reported in small-cell lung cancer (PMID: 25528583, 33524794). The observed molecular weight of L-Myc is 68 kDa, which is consistent with the description in the literature (PMID: 8657155).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for L-Myc antibody 14584-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The L-Myc Polyclonal antibody (Cat# 14584-1-AP) has 1 citation. It was used in Western Blot (WB) for human samples, published in Nature Cell Biology (IF 22.7, 2020). The study investigated how Merkel cell polyomavirus activates LSD1-mediated blockade of non-canonical BAF to regulate transformation and tumorigenesis, placing the research in the fields of virology, epigenetics, and cancer biology.
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Merkel cell polyomavirus activates LSD1-mediated blockade of non-canonical BAF to regulate transformation and tumorigenesis. |
Reviews
What customers say
The product has one verified review for Western Blot application. The reviewer rated it 4 out of 5 stars and reported that the antibody performed well at a 1:1000 dilution. Overall, the feedback is positive, indicating reliable performance for WB use.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sarah (Verified Customer) (10-27-2023) | Antibody worked well for western blot
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