Tested Applications
| Positive WB detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22004-1-AP targets MYCT1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4800 Product name: Recombinant human MYCT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-220 aa of BC022556 Sequence: LAVLQRDRIKLLFFDILVFLSVFLLFLLFLVDIMANNTTSLGSPWPENFWEDLIMSFTVSMAIGLVLGGFIWAVFICLSRRRRASAPISQWSSSRRSRSSYTHGLNRTGFYGHSGCERRSNLSLASLTFQRQASLEQANSFPRKSSFRASTFHPFLQCPPLPVETESQLVTLPSSNISPTISTSHSLSRPDYWSSNSLRVGLSTPPPPAYESIIKAFPDS Predict reactive species |
| Full Name | myc target 1 |
| Calculated Molecular Weight | 27 kDa |
| Observed Molecular Weight | 27-30 kDa |
| GenBank Accession Number | BC022556 |
| Gene Symbol | MYCT1 |
| Gene ID (NCBI) | 80177 |
| RRID | AB_2878968 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | Q8N699 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MYCT1 antibody 22004-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
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| Species | Application | Title |
|---|---|---|
J Extracell Vesicles Optimized AF4 combined with density cushion ultracentrifugation enables profiling of high-purity human blood extracellular vesicles | ||
Front Pharmacol Overexpression of MYCT1 Inhibits Proliferation and Induces Apoptosis in Human Acute Myeloid Leukemia HL-60 and KG-1a Cells in vitro and in vivo. | ||
J Cancer MUC15 is an independent prognostic factor that promotes metastases of MYCN non-amplified neuroblastoma |
Reviews
What customers say
The single review for this product (Western Blot, human retinal endothelial cells) reports a rating of 1 out of 5. The reviewer noted that no specific band was detected for endogenous MYCT1 after siRNA-mediated knockdown of Myct1, which achieved over 90% knockdown efficiency at the RNA level. This suggests the antibody failed to detect the target protein under the tested conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Zinnia (Verified Customer) (07-10-2024) | No specific band was detected for endogenous MYCT1 after using siRNA to knockdown Myct1 (more than 90% knockdown efficiency at RNA level)
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