MYEF2 Polyclonal antibody

MYEF2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 16051-1-AP

Synonyms

HsT18564, KIAA1341, MEF 2, MST156, MSTP156, MyEF 2, MYEF2, myelin expression factor 2



Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, HepG2 cells, MCF-7 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16051-1-AP targets MYEF2 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYEF2 fusion protein Ag9010
Full Name myelin expression factor 2
Calculated Molecular Weight 600 aa, 64 kDa
Observed Molecular Weight61-64 kDa
GenBank Accession NumberBC014533
Gene Symbol MYEF2
Gene ID (NCBI) 50804
RRIDAB_2146869
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MYEF2 antibody 16051-1-APDownload protocol
IHC protocol for MYEF2 antibody 16051-1-APDownload protocol
IF protocol for MYEF2 antibody 16051-1-APDownload protocol
IP protocol for MYEF2 antibody 16051-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB, IHC, CoIP

Cell Rep

PARP1-mediated PARylation activity is essential for oligodendroglial differentiation and CNS myelination.

Authors - Yan Wang
humanWB,IHC

BMC Gastroenterol

Expression and function of myelin expression factor 2 in hepatocellular carcinoma

Authors - Peng Zhang
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Wael (Verified Customer) (05-16-2023)

Hek293 cells were fractionated into nuclear and cytoplasmic fractions. specific band for MYEF2 between 55 and 100kd, enriched in the Nuclear fraction. Lanes order: 1-ladder 2-Nuclear fraction 3-Cytoplasmic frcation

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Hek293
MYEF2 Antibody Western Blot validation (1:1000 dilution) in Hek293 (Cat no:16051-1-AP)