Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | C2C12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11754-1-AP targets MYF6 in WB, IHC, IF/ICC, chip, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, chicken, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2350 Product name: Recombinant human MYF6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-242 aa of BC017834 Sequence: MMMDLFETGSYFFYLDGENVTLQPLEVAEGSPLYPGSDGTLSPCQDQMPPEAGSDSSGEEHVLAPPGLQPPHCPGQCLIWACKTCKRKSAPTDRRKAATLRERRRLKKINEAFEALKRRTVANPNQRLPKVEILRSAISYIERLQDLLHRLDQQEKMQELGVDPFSYRPKQENLEGADFLRTCSSQWPSVSDHSRGLVITAKEGGASIDSSASSSLRCLSSIVDSISSEERKLPCVEEVVEK Predict reactive species |
| Full Name | myogenic factor 6 (herculin) |
| Calculated Molecular Weight | 242 aa, 27 kDa |
| Observed Molecular Weight | 27 kDa |
| GenBank Accession Number | BC017834 |
| Gene Symbol | MYF6 |
| Gene ID (NCBI) | 4618 |
| RRID | AB_2250929 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P23409 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MYF6 antibody 11754-1-AP | Download protocol |
| IHC protocol for MYF6 antibody 11754-1-AP | Download protocol |
| WB protocol for MYF6 antibody 11754-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MYF6 Polyclonal antibody (Cat# 11754-1-AP) has 8 citations published in journals including Nature Communications, Nutrients, Frontiers in Cell and Developmental Biology, Poultry Science, Heliyon, BMC Genomics, Stem Cells International, and In Vitro Cell & Developmental Biology–Animal. The research fields span skeletal muscle growth regulation, myoblast senescence, satellite cell differentiation, adolescent idiopathic scoliosis, meat tenderness genetics, hydrogen sulfide effects on myogenic differentiation, and skeletal muscle regeneration.
| Species | Application | Title |
|---|---|---|
Nat Commun MRF4 negatively regulates adult skeletal muscle growth by repressing MEF2 activity. | ||
Nutrients Activation of Sirtuin3 by 6,4'-Dihydroxy-7-methoxyflavanone Against Myoblasts Senescence by Attenuating D-Galactose-Induced Oxidative Stress and Inflammation. | ||
Front Cell Dev Biol A Functional SNP in the Promoter of LBX1 Is Associated With the Development of Adolescent Idiopathic Scoliosis Through Involvement in the Myogenesis of Paraspinal Muscles. | ||
Poult Sci Dysfunctional terminal differentiation of satellite cells in the wooden breast muscle of Ross 308 broiler chickens | ||
Heliyon Exogenous hydrogen sulfide enhances myogenic differentiation of C2C12 myoblasts under high palmitate stress | ||
BMC Genomics Mining candidate genes for meat tenderness in Qinchuan cattle based on transcriptome analysis. |
Reviews
What customers say
The single review for 11754-1-AP is from Kenzo Oikawa (November 2023), who rated the product 4 out of 5 stars. He used it at a 1:200 dilution for immunofluorescence on frozen mouse heart tissue sections and reported that it "worked well." Overall, customer feedback is positive, with the antibody performing reliably in IF applications on mouse cardiac tissue.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Kenzo (Verified Customer) (11-11-2023) | Worked well for frozen mouse heart tissue sections.
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