Tested Applications
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse skeletal muscle tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25182-1-AP targets MYH1 (N-terminal) in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, pig, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17129 Product name: Recombinant human MYH1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-350 aa of BC114545 Sequence: MSSDSEMAIFGEAAPFLRKSERERIEAQNKPFDAKTSVFVVDPKESFVKATVQSREGGKVTAKTEAGATVTVKDDQVFPMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNAEVVTAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEVTSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQIMSNKKPDLIEMLLITTNPYDYAFVSQGEITVPSIDDQEELMATDSAIEILGFTSDERV Predict reactive species |
| Full Name | myosin, heavy chain 1, skeletal muscle, adult |
| Calculated Molecular Weight | 1939 aa, 223 kDa |
| Observed Molecular Weight | 220-230 kDa |
| GenBank Accession Number | BC114545 |
| Gene Symbol | MYH1 |
| Gene ID (NCBI) | 4619 |
| RRID | AB_2879947 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P12882 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MYH1 (MyHC-2x) encodes the IIX isoform of myosin heavy chain (MyHC). Myosin is a large, ubiquitous, motor protein that generates force through its interaction with actin, thus involving it in a number of cellular processes including cytokinesis, karyokinesis, cell migration, and muscle contraction. Muscle fibers can be divided as type 1 (slow) and type 2 (fast). MYH1 belongs to type 2.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MYH1 (N-terminal) antibody 25182-1-AP | Download protocol |
| IHC protocol for MYH1 (N-terminal) antibody 25182-1-AP | Download protocol |
| IP protocol for MYH1 (N-terminal) antibody 25182-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MYH1 (N-terminal) polyclonal antibody (Cat# 25182-1-AP) has 24 citations published in journals such as Materials Today Bio, J Agric Food Chem, Int J Mol Sci, Poult Sci, Sci Rep, Cells, Food Funct, Gene, Front Genet, PLoS One, Spine J, Int J Biol Macromol, J Muscle Res Cell Motil, Br Poult Sci, Heliyon, Genes, and Food Chem X. Research fields include skeletal muscle development, myoblast proliferation and differentiation, muscle fiber type regulation, myogenic signaling pathways, miRNA-mediated muscle gene regulation, and muscle aging.
| Species | Application | Title |
|---|---|---|
Mater Today Bio Conductive MeCbl/PEDOT:PSS/HA hydrogels with electrical stimulation for enhanced peripheral nerve regeneration | ||
Food Chem X Astaxanthin and naringin synergistically promote proliferation and myogenic differentiation of piscine satellite cells via mTOR signaling. | ||
Int J Biol Macromol Eicosapentaenoic acid-mediated activation of PGAM2 regulates skeletal muscle growth and development via the PI3K/AKT pathway | ||
J Agric Food Chem Single-Nucleus RNA Sequencing Reveals Cellular Heterogeneity and Trajectories of Lineage Differentiation during Chicken Skeletal Muscle Development. | ||
J Agric Food Chem Naringin Promotes Skeletal Muscle Fiber Remodeling by the AdipoR1-APPL1-AMPK Signaling Pathway. | ||
J Agric Food Chem Spermidine-Activated Satellite Cells Are Associated with Hypoacetylation in ACVR2B and Smad3 Binding to Myogenic Genes in Mice. |
