MYH10-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

MYH10-Specific Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Publications

9

Reviews (1)

Cat no : 19673-1-AP

Synonyms

MYH10, MYH10-Specific, Myosin 10, Myosin heavy chain 10

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Validation Data Gallery

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  WB analysis using 19673-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19673-1-AP (MYH10-Specific antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 19673-1-AP

  WB result of MYH10-Specific antibody (19673-1-AP; 1:80000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYH10-Specific transfected HEK-293 cells.

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  IP experiment of mouse brain using 19673-1-AP

  IP result of anti-MYH10-Specific (IP:19673-1-AP, 4ug; Detection:19673-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human cerebellum using 19673-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 19673-1-AP (MYH10-Specific Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human cerebellum using 19673-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 19673-1-AP (MYH10-Specific Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human brain using 19673-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 19673-1-AP (MYH10-Specific Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 19673-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 19673-1-AP (MYH10-Specific Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 19673-1-AP

  Immunofluorescent analysis of HepG2 cells, using MYH10 antibody 19673-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HepG2 using 19673-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MYH10-Specific antibody (19673-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HepG2 using 19673-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug MYH10-Specific antibody (19673-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: HEK-293T cells, HEK-293 cells, HeLa cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human cerebellum tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below

Product Information

19673-1-AP targets MYH10-Specific in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: myosin, heavy chain 10, non-muscle
• Calculated Molecular Weight: 231 kDa
• Observed Molecular Weight: 229 kDa
• GenBank Accession Number: NM_005964
• Gene Symbol: MYH10
• Gene ID (NCBI): 4628
• RRID: AB_10858231
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYH10 is cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. The antibody is specific to the C-term of MYH10.

Publications

Species	Application	Title
human	WB	Sci Adv; p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.; Authors - Xiaochao Tan
rat	WB,CoIP	Bioact Mater; Nidogen1-enriched extracellular vesicles accelerate angiogenesis and bone regeneration by targeting Myosin-10 to regulate endothelial cell adhesion.; Authors - Pengzhen Cheng
human	WB	J Am Heart Assoc; Magnesium Sulfate-Mediated Vascular Relaxation and Calcium Channel Activity in Placental Vessels Different From Nonplacental Vessels.; Authors - Jiaqi Tang
human	IHC	Clin Genet; Mutations of MYH14 Are Associated to Anorectal Malformations with Recto-perineal Fistulas in a Small Subset of Chinese Population.; Authors - Zhongxian Zhu
mouse	WB	Sci Rep; Angiotensin II-mediated MYH9 downregulation causes structural and functional podocyte injury in diabetic kidney disease.; Authors - Jeong Suk Kang
human	WB	J Dermatol Sci; Decreased mitochondrial function in UVA-irradiated dermal fibroblasts causes the insufficient formation of type I collagen and fibrillin-1 fibers; Authors - Yushi Katsuyama

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Ryan (Verified Customer) (02-27-2019)

Fish had been fixed in PFA then preserved in methanol, goat serum blocking solution, 1 in 200 dilution of Myh10 and 1 in 200 dilution of Cy3 secondary and no other treatments were necessary

• Applications: Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Zebrafish Heart

MYH10-Specific Antibody Immunohistochemistry,Immunofluorescence, validation (1:200 dilution) in Zebrafish Heart (Cat no:19673-1-AP)