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MYH10-Specific Polyclonal antibody

MYH10-Specific Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 19673-1-AP


MYH10, MYH10-Specific, Myosin 10, Myosin heavy chain 10

Tested Applications

Positive WB detected inHEK-293T cells, HEK-293 cells, HeLa cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman cerebellum tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19673-1-AP targets MYH10-Specific in WB, IP, IF, FC, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name myosin, heavy chain 10, non-muscle
Calculated Molecular Weight 231 kDa
Observed Molecular Weight 229 kDa
GenBank Accession NumberNM_005964
Gene Symbol MYH10
Gene ID (NCBI) 4628
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYH10 is cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. The antibody is specific to the C-term of MYH10.


Product Specific Protocols
WB protocol for MYH10-Specific antibody 19673-1-APDownload protocol
IHC protocol for MYH10-Specific antibody 19673-1-APDownload protocol
IF protocol for MYH10-Specific antibody 19673-1-APDownload protocol
IP protocol for MYH10-Specific antibody 19673-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Sci Adv

p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.

Authors - Xiaochao Tan

Bioact Mater

Nidogen1-enriched extracellular vesicles accelerate angiogenesis and bone regeneration by targeting Myosin-10 to regulate endothelial cell adhesion.

Authors - Pengzhen Cheng

J Am Heart Assoc

Magnesium Sulfate-Mediated Vascular Relaxation and Calcium Channel Activity in Placental Vessels Different From Nonplacental Vessels.

Authors - Jiaqi Tang

Clin Genet

Mutations of MYH14 Are Associated to Anorectal Malformations with Recto-perineal Fistulas in a Small Subset of Chinese Population.

Authors - Zhongxian Zhu

Sci Rep

Angiotensin II-mediated MYH9 downregulation causes structural and functional podocyte injury in diabetic kidney disease.

Authors - Jeong Suk Kang

J Dermatol Sci

Decreased mitochondrial function in UVA-irradiated dermal fibroblasts causes the insufficient formation of type I collagen and fibrillin-1 fibers

Authors - Yushi Katsuyama


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Ryan (Verified Customer) (02-27-2019)

Fish had been fixed in PFA then preserved in methanol, goat serum blocking solution, 1 in 200 dilution of Myh10 and 1 in 200 dilution of Cy3 secondary and no other treatments were necessary

  • Applications: Immunohistochemistry, Immunofluorescence,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Zebrafish Heart
MYH10-Specific Antibody Immunohistochemistry,Immunofluorescence, validation (1:200 dilution) in Zebrafish Heart (Cat no:19673-1-AP)