Tested Applications
| Positive WB detected in | HEK-293T cells, HEK-293 cells, HeLa cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human cerebellum tissue, human brain tissue, mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19673-1-AP targets MYH10-Specific in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | myosin, heavy chain 10, non-muscle |
| Calculated Molecular Weight | 231 kDa |
| Observed Molecular Weight | 229 kDa |
| GenBank Accession Number | NM_005964 |
| Gene Symbol | MYH10 |
| Gene ID (NCBI) | 4628 |
| RRID | AB_10858231 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P35580 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MYH10 is cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. The antibody is specific to the C-term of MYH10.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MYH10-Specific antibody 19673-1-AP | Download protocol |
| IHC protocol for MYH10-Specific antibody 19673-1-AP | Download protocol |
| IP protocol for MYH10-Specific antibody 19673-1-AP | Download protocol |
| WB protocol for MYH10-Specific antibody 19673-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Bioact Mater Nidogen1-enriched extracellular vesicles accelerate angiogenesis and bone regeneration by targeting Myosin-10 to regulate endothelial cell adhesion. | ||
J Am Heart Assoc Magnesium Sulfate-Mediated Vascular Relaxation and Calcium Channel Activity in Placental Vessels Different From Nonplacental Vessels. | ||
Sci Rep Disulfidptosis links the pathophysiology of ulcerative colitis and immune infiltration in colon adenocarcinoma | ||
Clin Genet Mutations of MYH14 Are Associated to Anorectal Malformations with Recto-perineal Fistulas in a Small Subset of Chinese Population. | ||
Exp Neurol ETAR silencing ameliorated neurovascular injury after SAH in rats through ERK/KLF4-mediated phenotypic transformation of smooth muscle cells |
Reviews
What customers say
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Ryan (Verified Customer) (02-27-2019) | Fish had been fixed in PFA then preserved in methanol, goat serum blocking solution, 1 in 200 dilution of Myh10 and 1 in 200 dilution of Cy3 secondary and no other treatments were necessary
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