MYH10-Specific Polyclonal antibody

MYH10-Specific Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 19673-1-AP

Synonyms

MYH10, Myosin 10, Myosin heavy chain 10



Tested Applications

Positive WB detected inHeLa cells, human brain tissue, SKOV-3 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman cerebellum tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

19673-1-AP targets MYH10-Specific in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name myosin, heavy chain 10, non-muscle
Calculated molecular weight 231 kDa
Observed molecular weight 229 kDa
GenBank accession numberNM_005964
Gene symbol MYH10
Gene ID (NCBI) 4628
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MYH10 is cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. The antibody is specific to the C-term of MYH10.

Protocols

Product Specific Protocols
WB protocol for MYH10-Specific antibody 19673-1-APDownload protocol
IHC protocol for MYH10-Specific antibody 19673-1-APDownload protocol
IF protocol for MYH10-Specific antibody 19673-1-APDownload protocol
IP protocol for MYH10-Specific antibody 19673-1-APDownload protocol
FC protocol for MYH10-Specific antibody 19673-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Sci Rep

Angiotensin II-mediated MYH9 downregulation causes structural and functional podocyte injury in diabetic kidney disease.

Authors - Jeong Suk Kang
humanIHC

Clin Genet

Mutations of MYH14 Are Associated to Anorectal Malformations with Recto-perineal Fistulas in a Small Subset of Chinese Population.

Authors - Zhongxian Zhu
humanWB

J Am Heart Assoc

Magnesium Sulfate-Mediated Vascular Relaxation and Calcium Channel Activity in Placental Vessels Different From Nonplacental Vessels.

Authors - Jiaqi Tang
humanWB

Sci Adv

p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.

Authors - Xiaochao Tan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Ryan (Verified Customer) (02-27-2019)

Fish had been fixed in PFA then preserved in methanol, goat serum blocking solution, 1 in 200 dilution of Myh10 and 1 in 200 dilution of Cy3 secondary and no other treatments were necessary

  • Applications: Immunohistochemistry,Immunofluorescence,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Zebrafish Heart
MYH10-Specific Antibody Immunohistochemistry,Immunofluorescence, validation (1:200 dilution) in Zebrafish Heart (Cat no:19673-1-AP)