Tested Applications
| Positive WB detected in | HEK-293T cells, HEK-293 cells, HeLa cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human cerebellum tissue, human brain tissue, mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19673-1-AP targets MYH10-Specific in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | myosin, heavy chain 10, non-muscle |
| Calculated Molecular Weight | 231 kDa |
| Observed Molecular Weight | 229 kDa |
| GenBank Accession Number | NM_005964 |
| Gene Symbol | MYH10 |
| Gene ID (NCBI) | 4628 |
| RRID | AB_10858231 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P35580 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MYH10 is cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. The antibody is specific to the C-term of MYH10.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MYH10-Specific antibody 19673-1-AP | Download protocol |
| IHC protocol for MYH10-Specific antibody 19673-1-AP | Download protocol |
| IP protocol for MYH10-Specific antibody 19673-1-AP | Download protocol |
| WB protocol for MYH10-Specific antibody 19673-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MYH10-Specific Polyclonal Antibody (Cat# 19673-1-AP) has 26 citations published in journals including Nature, Science Advances, Advanced Science, Genome Biology, Molecular Medicine, iScience, Respiratory Research, and Journal of Neurochemistry. Research fields span virology, angiogenesis, oocyte development, cancer metastasis, lipid homeostasis, cardiomyopathy, disulfidptosis, diabetic nephropathy, vascular biology, neurovascular injury, dermatology, cell biology, endometriosis, flagellogenesis, and brain evolution.
| Species | Application | Title |
|---|---|---|
Nature TMEFF1 is a neuron-specific restriction factor for herpes simplex virus
| ||
Bioact Mater Nidogen1-enriched extracellular vesicles accelerate angiogenesis and bone regeneration by targeting Myosin-10 to regulate endothelial cell adhesion. | ||
Adv Sci (Weinh) Microfilament-Myosin II Regulates the Differentiation of Multinucleated Cysts into Oocytes and Influences Oocyte Developmental Potential in Mice. | ||
Genome Biol Systematic interrogation of functional genes underlying cholesterol and lipid homeostasis
| ||
Mol Med Identification and validation of differentially expressed disulfidptosis-related genes in hypertrophic cardiomyopathy |
Reviews
What customers say
The single review for this product is a 5-star rating. The user applied it to zebrafish heart tissue for immunohistochemistry and immunofluorescence at a 1:200 dilution. They reported that after fixing the fish in PFA and preserving in methanol, they used goat serum blocking, 1:200 primary antibody, and 1:200 Cy3 secondary, noting no additional treatments were necessary. Overall, the reviewer found the protocol straightforward and effective.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Ryan (Verified Customer) (02-27-2019) | Fish had been fixed in PFA then preserved in methanol, goat serum blocking solution, 1 in 200 dilution of Myh10 and 1 in 200 dilution of Cy3 secondary and no other treatments were necessary
 |
