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MYH10-Specific Polyclonal antibody
MYH10-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 19673-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293T cells, HEK-293 cells, HeLa cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human cerebellum tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:20000-1:100000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 9 publications below |
IHC | See 1 publications below |
IF | See 2 publications below |
IP | See 1 publications below |
ELISA | See 1 publications below |
CoIP | See 1 publications below |
Product Information
19673-1-AP targets MYH10-Specific in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | myosin, heavy chain 10, non-muscle |
Calculated Molecular Weight | 231 kDa |
Observed Molecular Weight | 229 kDa |
GenBank Accession Number | NM_005964 |
Gene Symbol | MYH10 |
Gene ID (NCBI) | 4628 |
RRID | AB_10858231 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MYH10 is cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. The antibody is specific to the C-term of MYH10.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for MYH10-Specific antibody 19673-1-AP | Download protocol |
IHC protocol for MYH10-Specific antibody 19673-1-AP | Download protocol |
IF protocol for MYH10-Specific antibody 19673-1-AP | Download protocol |
IP protocol for MYH10-Specific antibody 19673-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Bioact Mater Nidogen1-enriched extracellular vesicles accelerate angiogenesis and bone regeneration by targeting Myosin-10 to regulate endothelial cell adhesion. | ||
J Am Heart Assoc Magnesium Sulfate-Mediated Vascular Relaxation and Calcium Channel Activity in Placental Vessels Different From Nonplacental Vessels. | ||
Clin Genet Mutations of MYH14 Are Associated to Anorectal Malformations with Recto-perineal Fistulas in a Small Subset of Chinese Population. | ||
Sci Rep Angiotensin II-mediated MYH9 downregulation causes structural and functional podocyte injury in diabetic kidney disease. | ||
J Dermatol Sci Decreased mitochondrial function in UVA-irradiated dermal fibroblasts causes the insufficient formation of type I collagen and fibrillin-1 fibers |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Ryan (Verified Customer) (02-27-2019) | Fish had been fixed in PFA then preserved in methanol, goat serum blocking solution, 1 in 200 dilution of Myh10 and 1 in 200 dilution of Cy3 secondary and no other treatments were necessary
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