|Positive WB detected in||HeLa cells, human brain tissue, SKOV-3 cells|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human cerebellum tissue, human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
19673-1-AP targets MYH10-Specific in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Full Name||myosin, heavy chain 10, non-muscle|
|Calculated molecular weight||231 kDa|
|Observed molecular weight||229 kDa|
|GenBank accession number||NM_005964|
|Gene ID (NCBI)||4628|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
MYH10 is cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. The antibody is specific to the C-term of MYH10.
|Product Specific Protocols|
|WB protocol for MYH10-Specific antibody 19673-1-AP||Download protocol|
|IHC protocol for MYH10-Specific antibody 19673-1-AP||Download protocol|
|IF protocol for MYH10-Specific antibody 19673-1-AP||Download protocol|
|IP protocol for MYH10-Specific antibody 19673-1-AP||Download protocol|
|FC protocol for MYH10-Specific antibody 19673-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Ryan (Verified Customer) (02-27-2019)
Fish had been fixed in PFA then preserved in methanol, goat serum blocking solution, 1 in 200 dilution of Myh10 and 1 in 200 dilution of Cy3 secondary and no other treatments were necessary