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MYH10-Specific Polyclonal antibody
MYH10-Specific Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 19673-1-AP
Validation Data Gallery
|Positive WB detected in||HEK-293T cells, HEK-293 cells, HeLa cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human cerebellum tissue, human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:20000-1:100000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
19673-1-AP targets MYH10-Specific in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||myosin, heavy chain 10, non-muscle|
|Calculated molecular weight||231 kDa|
|Observed molecular weight||229 kDa|
|GenBank accession number||NM_005964|
|Gene ID (NCBI)||4628|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
MYH10 is cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. The antibody is specific to the C-term of MYH10.
|Product Specific Protocols|
|WB protocol for MYH10-Specific antibody 19673-1-AP||Download protocol|
|IHC protocol for MYH10-Specific antibody 19673-1-AP||Download protocol|
|IF protocol for MYH10-Specific antibody 19673-1-AP||Download protocol|
|IP protocol for MYH10-Specific antibody 19673-1-AP||Download protocol|
|Click here to view our Standard Protocols|
p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.
Nidogen1-enriched extracellular vesicles accelerate angiogenesis and bone regeneration by targeting Myosin-10 to regulate endothelial cell adhesion.
J Am Heart Assoc
Magnesium Sulfate-Mediated Vascular Relaxation and Calcium Channel Activity in Placental Vessels Different From Nonplacental Vessels.
Mutations of MYH14 Are Associated to Anorectal Malformations with Recto-perineal Fistulas in a Small Subset of Chinese Population.
Angiotensin II-mediated MYH9 downregulation causes structural and functional podocyte injury in diabetic kidney disease.
J Dermatol Sci
Decreased mitochondrial function in UVA-irradiated dermal fibroblasts causes the insufficient formation of type I collagen and fibrillin-1 fibers
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Ryan (Verified Customer) (02-27-2019)
Fish had been fixed in PFA then preserved in methanol, goat serum blocking solution, 1 in 200 dilution of Myh10 and 1 in 200 dilution of Cy3 secondary and no other treatments were necessary