SMMHC Polyclonal antibody

SMMHC Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 18569-1-AP

Synonyms

MYH11, Myosin 11, Myosin heavy chain 11, SMHC, SMMHC, SMM-HC, smooth muscle Myosin heavy chain

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Validation Data Gallery

• 

  WB analysis of mouse colon using 18569-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 18569-1-AP (SMMHC antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat uterus using 18569-1-AP

  rat uterus tissue were subjected to SDS PAGE followed by western blot with 18569-1-AP (SMMHC antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse uterus using 18569-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 18569-1-AP (SMMHC antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse bladder using 18569-1-AP

  mouse bladder tissue were subjected to SDS PAGE followed by western blot with 18569-1-AP (SMMHC antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse colon tissue, mouse bladder tissue, mouse uterus tissue, rat uterus tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below

Product Information

The immunogen of 18569-1-AP is SMMHC Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SMMHC fusion protein Ag13391
• Full Name: myosin, heavy chain 11, smooth muscle
• Calculated molecular weight: 1938 aa, 224 kDa
• Observed molecular weight: 227 kDa
• GenBank accession number: BC101677
• Gene symbol: MYH11
• Gene ID (NCBI): 4629
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Am Heart Assoc; LINC01278 Sponges miR-500b-5p to Regulate the Expression of ACTG2 to Control Phenotypic Switching in Human Vascular Smooth Muscle Cells During Aortic Dissection.; Authors - Weitie Wang
rat	WB	Front Pharmacol; Theaflavin-3,3'-Digallate from Black Tea Inhibits Neointima Formation Through Suppression of the PDGFRβ Pathway in Vascular Smooth Muscle Cells.; Authors - Yichen Wu
human	WB	Front Med (Lausanne); Depiction of Aging-Based Molecular Phenotypes With Diverse Clinical Prognosis and Immunological Features in Gastric Cancer.; Authors - Fang He
human	WB	Ultrasound Med Biol; 5-Aminolevulinic Acid-Mediated Sonodynamic Therapy Promotes Phenotypic Switching from Dedifferentiated to Differentiated Phenotype via Reactive Oxygen Species and p38 Mitogen-Activated Protein Kinase in Vascular Smooth Muscle Cells.; Authors - Juhua Dan
mouse	IHC	Cell Signal; Sphingosine 1-phosphate receptor 2 promotes erythrocyte clearance by vascular smooth muscle cells in intraplaque hemorrhage through MFG-E8 production.; Authors - Daorong Pan
human	WB	Mol Med Rep; miRNA‑92a inhibits vascular smooth muscle cell phenotypic modulation and may help prevent in‑stent restenosis; Authors - Fenfen Jiang

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