MYH3 Polyclonal antibody

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  WB analysis of mouse embryo using 22287-1-AP

  mouse embryo tissue were subjected to SDS PAGE followed by western blot with 22287-1-AP (MYH3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 22287-1-AP

  IP result of anti-MYH3 (IP:22287-1-AP, 4ug; Detection:22287-1-AP 1:300) with mouse skeletal muscle tissue lysate 4000ug.

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  IHC staining of mouse heart using 22287-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22287-1-AP (MYH3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 22287-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22287-1-AP (MYH3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat heart using 22287-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 22287-1-AP (MYH3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 22287-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 22287-1-AP (MYH3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MYH3 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 22287-1-AP

Publications(15)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

eMyHC, HEMHC, MYH3, MYHC EMB, MYHSE1, Myosin 3, Myosin heavy chain 3, SMHCE

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Tested Applications

• Positive WB detected in: mouse embryo tissue
• Positive IP detected in: mouse skeletal muscle tissue
• Positive IHC detected in: rat heart tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 11 publications below
• IF: See 6 publications below

Product Information

22287-1-AP targets MYH3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: myosin, heavy chain 3, skeletal muscle, embryonic
• Calculated Molecular Weight: 234 kDa
• Observed Molecular Weight: 220-230 kDa
• GenBank Accession Number: NM_002470
• Gene Symbol: MYH3
• Gene ID (NCBI): 4621
• RRID: AB_2879060
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P11055
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Nature; A non-canonical tricarboxylic acid cycle underlies cellular identity.; Authors - Paige K Arnold
rat	WB	Basic Res Cardiol; Alkaline nucleoplasm facilitates contractile gene expression in the mammalian heart.; Authors - Alzbeta Hulikova
mouse	IF	Cell Rep; Intramuscular adipose tissue restricts functional muscle recovery; Authors - Alessandra M Norris
mouse	WB	Int J Mol Sci; Molecular and Metabolic Mechanism of Low-Intensity Pulsed Ultrasound Improving Muscle Atrophy in Hindlimb Unloading Rats.; Authors - Lijun Sun
mouse	WB,IF	FASEB J; miR-206 family is important for mitochondrial and muscle function, but not essential for myogenesis in vitro.; Authors - Roza K Przanowska
		Am J Pathol; B4GALNT2 (GALGT2) Gene Therapy Reduces Skeletal Muscle Pathology in the FKRP P448L Mouse Model of Limb Girdle Muscular Dystrophy 2I.; Authors - Paul J Thomas

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Alessandra (Verified Customer) (03-23-2022)

Antigen retrieval was performed on Tibialis Anterior muscle cross sections. MYH3 in red (newly formed fibers); IgG in cyan (dying muscle fibers)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: Skeletal Muscle/Myoblast

MYH3 Antibody Immunofluorescence validation (1:300 dilution) in Skeletal Muscle/Myoblast (Cat no:22287-1-AP)