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MYH6 Monoclonal antibody

MYH6 Monoclonal Antibody for WB, ELISA

Cat No. 68461-1-Ig
Clone No. 1A5E5
Publications (2)
Review Product
Download datasheet

Host / Isotype

Mouse / IgG1

Reactivity

mouse, rat

Applications

WB, ELISA

Myosin 6, 1A5E5, MYHCA, MyHC-alpha, Myosin heavy chain 6

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 
SKU: 

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Freight/Packing: -

Quantity

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What's the best dilution for MYH6 in ?

Tested Applications

Positive WB detected inmouse heart tissue, rat heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68461-1-Ig targets MYH6 in WB, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Cited Reactivitymouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

Peptide

Predict reactive species
Full Name myosin, heavy chain 6, cardiac muscle, alpha
Calculated Molecular Weight 224 kDa
Observed Molecular Weight 200-220 kDa
GenBank Accession NumberNM_002471
Gene Symbol MYH6
Gene ID (NCBI) 4624
RRIDAB_3085173
Conjugate Unconjugated
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP13533
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MYH6 antibody 68461-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The MYH6 Monoclonal antibody (1A5E5), catalog number 68461-1-Ig, has a total of 2 citations. These appear in Cell Death Dis and Am J Physiol Heart Circ Physiol. The cited research fields include cardiac fibroblast maturation and paracrine signaling involving long non-coding RNA (Charme), as well as maternal cardiac remodeling associated with hypercholesterolemia during pregnancy. Both studies utilized the antibody for Western blot applications in mouse and rat models.

SpeciesApplicationTitle
mouseWB

Cell Death Dis

Effect of the cardiac long non-coding RNA Charme depletion on the maturation and paracrine signaling of resident cardiac fibroblasts.

Authors - Erica Floris
ratWB

Am J Physiol Heart Circ Physiol

Excessive hypercholesterolemia in pregnancy alters maternal cardiac remodeling during pregnancy and postpartum.

Authors - Amanda A. de Oliveira
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