MYH8-Specific Polyclonal antibody

MYH8-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18988-1-AP

Synonyms

MYH8, MYH8-Specific, MyHC peri, MyHC perinatal, MyHC pn, Myosin 8, Myosin heavy chain 8

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Validation Data Gallery

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  WB analysis using 18988-1-AP

  Various lysates were subjected to Tris-Acetate gel system followed by western blot with 18988-1-AP (MYH8-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 18988-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 18988-1-AP (MYH8-Specific antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue
• Positive IHC detected in: mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18988-1-AP targets MYH8-Specific in WB, IHC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: myosin, heavy chain 8, skeletal muscle, perinatal
• Calculated Molecular Weight: 223 kDa
• Observed Molecular Weight: 220-230 kDa
• GenBank Accession Number: NM_002472
• Gene Symbol: MYH8
• Gene ID (NCBI): 4626
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYH8 plays a role in muscle contraction. It is required for cytoskeleton organization. The antibody is specific to MYH8 and MYH2.