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  • KD/KO Validated

MYH9 Monoclonal antibody

MYH9 Monoclonal Antibody for IF, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

5D9D2

Cat no : 60233-1-Ig

Synonyms

DFNA17, EPSTS, FTNS, MHA, MYH9, Myosin 9, Myosin heavy chain 9



Tested Applications

Positive WB detected inmouse kidney tissue, human kidney tissue, pig kidney tissue, pig spleen tissue, human placenta tissue, pig pancreas tissue, A431 cells
Positive IF detected inHepG2 cells, U2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 60233-1-Ig is MYH9 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, pig
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MYH9 fusion protein Ag1612
Full Name myosin, heavy chain 9, non-muscle
Calculated molecular weight 227 kDa
Observed molecular weight 230 kDa, 160 kDa
GenBank accession numberBC011915
Gene symbol MYH9
Gene ID (NCBI) 4627
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MYH9 (Myosin-9) appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. MYH9 is a hexameric protein that consists of two heavy chains (~200 kDa) and two pairs of non-identical light chains (15-22 kDa), a pair of essential or alkali light chains, and a pair of regulatory light chains (PMID: 12800156).

Protocols

Product Specific Protocols
WB protocol for MYH9 antibody 60233-1-IgDownload protocol
IF protocol for MYH9 antibody 60233-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IF

EBioMedicine

PMID: 31594754

Authors - Yiyi Liu
  • KD Validated
humanIF

J Autoimmun

Antibodies in cerebral cavernous malformations react with cytoskeleton autoantigens in the lesional milieu.

Authors - Dongdong Zhang
mouseIF

Theranostics

Salvia miltiorrhiza-derived miRNAs suppress vascular remodeling through regulating OTUD7B/KLF4/NMHC IIA axis.

Authors - Gao-Shan Yang
humanWB

Nat Commun

Lymphocyte-specific protein 1 regulates mechanosensory oscillation of podosomes and actin isoform-based actomyosin symmetry breaking.

Authors - Pasquale Cervero