|Positive WB detected in||mouse kidney tissue, human kidney tissue, pig kidney tissue, pig spleen tissue, human placenta tissue, pig pancreas tissue, A431 cells|
|Positive IF detected in||HepG2 cells, U2OS cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 60233-1-Ig is MYH9 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, pig|
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||MYH9 fusion protein Ag1612|
|Full Name||myosin, heavy chain 9, non-muscle|
|Calculated molecular weight||227 kDa|
|Observed molecular weight||230 kDa, 160 kDa|
|GenBank accession number||BC011915|
|Gene ID (NCBI)||4627|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
MYH9 (Myosin-9) appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. MYH9 is a hexameric protein that consists of two heavy chains (~200 kDa) and two pairs of non-identical light chains (15-22 kDa), a pair of essential or alkali light chains, and a pair of regulatory light chains (PMID: 12800156).
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