MYL3 Monoclonal antibody

MYL3 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse, pig

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

2E4B2

Cat no : 66286-1-Ig

Synonyms

Cardiac myosin light chain 1, CMH8, CMLC1, MLC1SB, MLC1V, MYL3, Myosin light chain 3, VLC1



Tested Applications

Positive WB detected inhuman skeletal muscle tissue, mouse heart tissue
Positive IHC detected inhuman heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

66286-1-Ig targets MYL3 in WB, IHC,ELISA applications and shows reactivity with human, rat, mouse, pig samples.

Tested Reactivity human, rat, mouse, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MYL3 fusion protein Ag24535
Full Name myosin, light chain 3, alkali; ventricular, skeletal, slow
Calculated molecular weight 22 kDa
Observed molecular weight 22-25 kDa
GenBank accession numberBC009790
Gene symbol MYL3
Gene ID (NCBI) 4634
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MYL3, also named as MLC1v, is an essential light chain of myosin that is associated with muscle contraction. It is expressed in ventricular and slow skeletal muscle. MYL3 may serve as a target for caspase-3 in dying cardiomyocytes. Mutations of MYL3 gene cause hypertrophic cardiomyopathy. MYL3 has been identified as potential serum biomarker for drug induced myotoxicity. Great increase in MYL3 serum concentration has been observed in rats with cardiac and skeletal muscle injury. (PMID:21685905)

Protocols

Product Specific Protocols
WB protocol for MYL3 antibody 66286-1-IgDownload protocol
IHC protocol for MYL3 antibody 66286-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols