MYLIP Polyclonal antibody

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  WB analysis of human cerebellum using 15455-1-AP

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 15455-1-AP (MYLIP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of CHO using 15455-1-AP

  CHO cells were subjected to SDS PAGE followed by western blot with 15455-1-AP (MYLIP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 15455-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 15455-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 15455-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 15455-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 15455-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 15455-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 15455-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 15455-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 15455-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 15455-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung using 15455-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung using 15455-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 15455-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 15455-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 15455-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 15455-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 15455-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 15455-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 15455-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 15455-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 40x lens).

MYLIP Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15455-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, hamster

Applications

WB, IHC, ELISA

Synonyms

BZF1, Idol, MIR, MYLIP, PP5242

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Tested Applications

• Positive WB detected in: human cerebellum tissue, CHO cells
• Positive IHC detected in: human testis tissue, human brain tissue, human heart tissue, human kidney tissue, human liver tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

15455-1-AP targets MYLIP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, hamster samples.

• Tested Reactivity: human, mouse, rat, hamster
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MYLIP fusion protein Ag7720
• Full Name: myosin regulatory light chain interacting protein
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 46-50 kDa
• GenBank Accession Number: BC002860
• Gene Symbol: MYLIP
• Gene ID (NCBI): 29116
• RRID: AB_2235610
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8WY64
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Elife; SEC24A deficiency lowers plasma cholesterol through reduced PCSK9 secretion.; Authors - Chen Xiao-Wei XW
mouse	WB	Lett Appl Microbiol; Xinnaokang improves cecal microbiota and lipid metabolism to target atherosclerosis.; Authors - R Yang
human	WB	Heliyon; Identification of ubiquitin markers for survival and prognosis of ovarian cancer; Authors - Yiwen Feng