MYLK3 Polyclonal antibody

MYLK3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 21527-1-AP

Synonyms

Myosin light chain kinase 3, MLCK, EC:2.7.11.18, Cardiac-MyBP-C-associated Ca/CaM kinase, Cardiac-MLCK

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Validation Data Gallery

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  WB analysis using 21527-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21527-1-AP (MYLK3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 21527-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 21527-1-AP (MYLK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 21527-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 21527-1-AP (MYLK3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon using 21527-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 21527-1-AP (MYLK3 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human heart using 21527-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21527-1-AP (MYLK3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 21527-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21527-1-AP (MYLK3 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 21527-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MYLK3 antibody (21527-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 21527-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 21527-1-AP (MYLK3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse heart tissue, human heart tissue, rat heart tissue
• Positive IHC detected in: human colon tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

21527-1-AP targets MYLK3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MYLK3 fusion protein Ag16137
• Full Name: myosin light chain kinase 3
• Calculated Molecular Weight: 819 aa, 88 kDa
• Observed Molecular Weight: 85-100 kDa
• GenBank Accession Number: BC109097
• Gene Symbol: MYLK3
• Gene ID (NCBI): 91807
• RRID: AB_10734432
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cardiac myosin light chain kinase (cMLCK) encoded by the myosin light chain kinase 3 (MYLK3) phosphorylates cardiac myosin light chain 2 (MLC2, encoded by MYL2) and facilitates actin-myosin interactions that enhance contractile force in the animal heart (PMID: 29235529).

Publications

Species	Application	Title
rat	WB	Basic Res Cardiol; Alkaline nucleoplasm facilitates contractile gene expression in the mammalian heart.; Authors - Alzbeta Hulikova
human,mouse	IHC,WB,IF	Cell Commun Signal; GPR65 inhibits human trophoblast cell adhesion through upregulation of MYLK and downregulation of fibronectin via cAMP-ERK signaling in a low pH environment; Authors - Jia Mao
mouse	WB	Nat Commun; Capture of RNA-binding proteins across mouse tissues using HARD-AP; Authors - Yijia Ren