MYO1C Polyclonal antibody

MYO1C Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17969-1-AP

Synonyms

MMI beta, MMIb, MYO1C, Myosin I beta, myosin IC, myr2

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Validation Data Gallery

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  IHC staining of human colon cancer using 17969-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17969-1-AP (MYO1C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 17969-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17969-1-AP (MYO1C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 17969-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 17969-1-AP (MYO1C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 17969-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 17969-1-AP (MYO1C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 17969-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 17969-1-AP (MYO1C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 17969-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 17969-1-AP (MYO1C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U2OS using 17969-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using MYO1C antibody (17969-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human kidney tissue, human colon cancer tissue, mouse colon tissue, rat colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: U2OS cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17969-1-AP targets MYO1C in IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MYO1C fusion protein Ag11970
• Full Name: myosin IC
• Calculated Molecular Weight: 1028 aa, 118 kDa
• GenBank Accession Number: BC068013
• Gene Symbol: MYO1C
• Gene ID (NCBI): 4641
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.